tailieunhanh - Báo cáo y học: "Deletion of 1.8-kb mRNA of Marek’s disease virus decreases its replication ability but not oncogenicity"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Deletion of mRNA of Marek’s disease virus decreases its replication ability but not oncogenicity | Sun et al. Virology Journal 2010 7 294 http content 7 1 294 VIROLOGY JOURNAL RESEARCH Open Access Deletion of mRNA of Marek s disease virus decreases its replication ability but not oncogenicity A 1 2 1 1 3 2 4 71 I z- 1 Aijun Sun T Yanpeng Li T Jingyan Wang Shuai Su Hongjun Chen Hongfei Zhu Jiabo Ding Zhizhong Cui Abstract Background The mRNA was reported as one of the oncogenesis-related genes of Marek s disease virus MDV . In this study the bacterial artificial chromosome BAC clone of a MDV field strain GX0101 was used as the platform to generate mutant MDV to examine the functional roles of mRNA. Results Based on the BAC clone of GX0101 the mRNA deletion mutant GX0101A A C was constructed. The present experiments indicated that GX0101A A C retained a low level of oncogenicity and it showed a decreased replication capacity in vitro and in vivo when compared with its parent virus GX0101. Further studies in vitro demonstrated that deletion of mRNA significantly decreased the transcriptional activity of the bidirectional promoter between mRNA and pp38 genes of MDV. Conclusion These results suggested that the mRNA did not directly influence the oncogenesis but related to the replication ability of MDV. Background Marek s disease MD is a contagious lymphoproliferative disease of poultry caused by the highly oncogenic alphaherpesvirus MDV which is characteristic by mononuclear infiltration of peripheral nerves irises skin and other visceral tissues 1 2 . Among the 100 genes encoded by MDV three genes including mRNA pp38 and meq were considered to be associated with oncogenicity of MDV serotype 1 and they are also unique to MDV 3 4 . Previous studies suggested that meq is involved in lymphocyte transformation 5 6 and pp38 is involved in early cytolytic infection in lymphocytes but not in the induction of tumors 7 . In addition recent studies indicated that pp38 could also enhance the activity of