tailieunhanh - Báo cáo y học: " Prevalence of hepatitis delta virus infection among hepatitis b virus surface antigen positive patients circulating in the largest province of pakistan"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Prevalence of hepatitis delta virus infection among hepatitis b virus surface antigen positive patients circulating in the largest province of pakistan | Zaidi et al. Virology Journal 2010 7 283 http content 7 1 283 VIROLOGY JOURNAL RESEARCH Open Access Prevalence of hepatitis delta virus infection among hepatitis b virus surface antigen positive patients circulating in the largest province of pakistan 1 2 3 2 2 r- 2 Gulshan Zaidi Muhammad Idrees Fayyaz Ahmed Malik Irum Amin Muhammad Shahid Saima Younas Rashid Hussain1 Zunaira Awan2 Aaliyah Tariq2 Khalida Parveen1 Abstract Background Hepatitis delta virus HDV and Hepatitis B virus HBV co-infection is well known to induce a spectrum of acute and chronic liver diseases which further advance to cirrhosis fulminant hepatitis and hepatocellular carcinoma HCC . Aim The aim of the present study was to determine the prevalence of hepatitis D virus super-infection among hepatitis B surface antigen HBsAg positive individuals in the highly populated province of Pakistan which is not well known. Methods Sera samples were subjected to HBsAg and anti-HDV screening and finally anti-HDV and HBsAg positive coinfected samples were used for HDV active RNA confirmation using nested polymerase chain reaction PCR . Results Out of total 200 HBsAg positive samples by rapid device 96 48 were also found reactive for HBsAg using enzyme linked immunosorbant assay ELISA . Out of these HBsAg ELISA positive samples 80 were anti-HDV ELISA positive which were then subjected to PCR. The amplification results further confirmed 24 30 samples to be HDV RNA positive. HDV super-infection was more common in male patients than female patients 81 VS 19 . Conclusion The current study shows a high prevalence rate of HDV-HBV co-infection in Pakistan that tends to increase over time. Background More than 350 million individuals worldwide are Hepatitis B Virus HBV carriers and at least 5 of these are co-infected with hepatitis delta virus HDV 1 . HDV is a delta agent that is deformed and incomplete RNA virus whose replication and expression is dependent on the presence of HBsAg. HDV is .