tailieunhanh - Báo cáo y học: " MicroRNA profiling of tomato leaf curl new delhi virus (tolcndv) infected tomato leaves indicates that deregulation of mir159/319 and mir172 might be linked with leaf curl disease"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: MicroRNA profiling of tomato leaf curl new delhi virus (tolcndv) infected tomato leaves indicates that deregulation of mir159/319 and mir172 might be linked with leaf curl disease | Naqvi et al. Virology Journal 2010 7 281 http content 7 1 281 VIROLOGY JOURNAL RESEARCH Open Access MicroRNA profiling of tomato leaf curl new delhi virus tolendv infected tomato leaves indicates that deregulation of mir159 319 and mir172 might be linked with leaf curl disease Afsar R Naqvi1 2 Qazi MR Haq2 Sunil K Mukherjee 1 Abstract Background Tomato leaf curl virus ToLCV a constituent of the genus Begomovirus infects tomato and other plants with a hallmark disease symptom of upward leaf curling. Since microRNAs miRs are known to control plants developmental processes we evaluated the roles of miRNAs in Tomato leaf curl New Delhi virus ToLCNDV induced leaf curling. Results Microarray analyses of miRNAs isolated from the leaves of both healthy and ToLCNDV agroinfected tomato cv Pusa Ruby revealed that ToLCNDV infection significantly deregulated various miRNAs representing 13 different conserved families . miR319 miR172 etc. . The precursors of these miRNAs showed similar deregulated patterns indicating that the transcription regulation of respective miRNA genes was perhaps the cause of deregulation. The expression levels of the miRNA-targeted genes were antagonistic with respect to the amount of corresponding miRNA. Such deregulation was tissue-specific in nature as no analogous misexpression was found in flowers. The accumulation of miR159 319 and miR172 was observed to increase with the days post inoculation dpi of ToLCNDV agroinfection in tomato cv Pusa Ruby. Similarly these miRs were also induced in ToLCNDV agroinfected tomato cv JK Asha and chilli plants both exhibiting leaf curl symptoms. Our results indicate that miR159 319 and miR172 might be associated with leaf curl symptoms. This report raises the possibility of using miRNA s as potential signature molecules for ToLCNDV infection. Conclusions The expression of several host miRNAs is affected in response to viral infection. The levels of the corresponding pre-miRs and the predicted .