tailieunhanh - Báo cáo y học: "Lassa virus-like particles displaying all major immunological determinants as a vaccine candidate for Lassa hemorrhagic fever"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Lassa virus-like particles displaying all major immunological determinants as a vaccine candidate for Lassa hemorrhagic fever | Branco et al. Virology Journal 2010 7 279 http content 7 1 279 VIROLOGY JOURNAL RESEARCH Open Access Lassa virus-like particles displaying all major immunological determinants as a vaccine candidate for Lassa hemorrhagic fever 1 3 3 3 4 Luis M Branco 1 Jessica N Grove Frederick J Geske Matt L Boisen Ivana J Muncy Susan A Magliato Lee A Henderson5 Randal J Schoepp6 Kathleen A Cashman7 Lisa E Hensley7 Robert F Garry1 Abstract Background Lassa fever is a neglected tropical disease with significant impact on the health care system society and economy of Western and Central African nations where it is endemic. Treatment of acute Lassa fever infections has successfully utilized intravenous administration of ribavirin a nucleotide analogue drug but this is not an approved use efficacy of oral administration has not been demonstrated. To date several potential new vaccine platforms have been explored but none have progressed toward clinical trials and commercialization. Therefore the development of a robust vaccine platform that could be generated in sufficient quantities and at a low cost per dose could herald a subcontinent-wide vaccination program. This would move Lassa endemic areas toward the control and reduction of major outbreaks and endemic infections. To this end we have employed efficient mammalian expression systems to generate a Lassa virus LASV -like particle VLP -based modular vaccine platform. Results A mammalian expression system that generated large quantities of LASV VLP in human cells at small scale settings was developed. These VLP contained the major immunological determinants of the virus glycoprotein complex nucleoprotein and Z matrix protein with known post-translational modifications. The viral proteins packaged into LASV VLP were characterized including glycosylation profiles of glycoprotein subunits GP1 and GP2 and structural compartmentalization of each polypeptide. The host cell protein component of LASV VLP was also .