tailieunhanh - Báo cáo y học: "Identification and characterization of duck plague virus glycoprotein C gene and gene product"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Identification and characterization of duck plague virus glycoprotein C gene and gene product | Lian et al. Virology Journal 2010 7 349 http content 7 1 349 VIROLOGY JOURNAL RESEARCH Open Access Identification and characterization of duck plague virus glycoprotein C gene and gene product 1 7 1 1 12 2 2 Bei Lian Chao Xu Anchun Cheng Mingshu Wang Dekang Zhu Qihui Luo Renyong Jia Fengjun Bi 2 Zhengli Chen2 Yi Zhou2 Zexia Yang2 Xiaoyue Chen1 2 3 Abstract Background Viral envelope proteins have been proposed to play significant roles in the process of viral infection. Results In this study an envelope protein gene gC NCBI GenBank accession no. EU076811 was expressed and characterized from duck plague virus DPV a member of the family herpesviridae. The gene encodes a protein of 432 amino acids with a predicted molecular mass of 45 kDa. Sequence comparisons multiple alignments and phylogenetic analysis showed that DPV gC has several features common to other identified herpesvirus gC and was genetically close to the gallid herpervirus. Antibodies raised in rabbits against the pET32a-gC recombinant protein expressed in Escherichia coli BL21 DE3 recognized a 45-KDa DPV-specific protein from infected duck embryo fibroblast DEF cells. Transcriptional and expression analysis using real-time fluorescent quantitative PCR FQ-PCR and Western blot detection revealed that the transcripts encoding DPV gC and the protein itself appeared late during infection of DEF cells. Immunofluorescence localization further demonstrated that the gC protein exhibited substantial cytoplasm fluorescence in DPV-infected DEF cells. Conclusions In this work the DPV gC protein was successfully expressed in a prokaryotic expression system and we presented the basic properties of the DPV gC product for the first time. These properties of the gC protein provided a prerequisite for further functional analysis of this gene. Background Duck plague virus DPV or duck enteritis virus DEV is an important pathogen of ducks which has caused serious losses in commercial duck .