tailieunhanh - Báo cáo y học: " Analysis of a new strain of Euphorbia mosaic virus with distinct replication specificity unveils a lineage of begomoviruses with short Rep sequences in the DNA-B intergenic region"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Analysis of a new strain of Euphorbia mosaic virus with distinct replication specificity unveils a lineage of begomoviruses with short Rep sequences in the DNA-B intergenic region | Gregorio-Jorge et al. Virology Journal 2010 7 275 http content 7 1 275 VIROLOGY JOURNAL RESEARCH Open Access Analysis of a new strain of Euphorbia mosaic virus with distinct replication specificity unveils a lineage of begomoviruses with short Rep sequences in the DNA-B intergenic region 1 f 2f 1 1 Josefat Gregorio-Jorge Artemiza Bernal-Alcocer Bernardo Banuelos-Hernandez Angel G Alpuche-Solís Cecilia Hernández-Zepeda3 Oscar Moreno-Valenzuela3 Gustavo Frias-Trevino2 Gerardo R Arguello-Astorga 1 Abstract Background Euphorbia mosaic virus EuMV is a member of the SLCV clade a lineage of New World begomoviruses that display distinctive features in their replication-associated protein Rep and virion-strand replication origin. The first entirely characterized EuMV isolate is native from Yucatan Peninsula Mexico subsequently EuMV was detected in weeds and pepper plants from another region of Mexico and partial DNA-A sequences revealed significant differences in their putative replication specificity determinants with respect to EuMV-YP. This study was aimed to investigate the replication compatibility between two EuMV isolates from the same country. Results A new isolate of EuMV was obtained from pepper plants collected at Jalisco Mexico. Full-length clones of both genomic components of EuMV-Jal were biolistically inoculated into plants of three different species which developed symptoms indistinguishable from those induced by EuMV-YP. Pseudorecombination experiments with EuMV-Jal and EuMV-YP genomic components demonstrated that these viruses do not form infectious reassortants in Nicotiana benthamiana presumably because of Rep-iteron incompatibility. Sequence analysis of the EuMV-Jal DNA-B intergenic region IR led to the unexpected discovery of a 35-nt-long sequence that is identical to a segment of the rep gene in the cognate viral DNA-A. Similar short rep sequences ranging from 35- to 51-nt in length were identified in all EuMV isolates and in three .