tailieunhanh - Báo cáo y học: "Porcine circovirus type 2 (PCV2): genetic variation and newly emerging genotypes in China"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Porcine circovirus type 2 (PCV2): genetic variation and newly emerging genotypes in China | Guo et al. Virology Journal 2010 7 273 http content 7 1 273 VIROLOGY JOURNAL RESEARCH Open Access Porcine circovirus type 2 PCV2 genetic variation and newly emerging genotypes in China Long J Guo Yue H Lu Yan W Wei Li P Huang Chang M Liu Abstract Background Porcine circovirus type 2 PCV2 the causative agent of postweaning multisystemic wasting syndrome PMWS is a serious economic problem for the swine industry in China. In this study we investigated the genetic variation of PCV2 in China using strains isolated from 2004-2008. Viruses were isolated from samples collected from pigs with multi-systemic lesions and clinical signs of PMWS from different regions of China and the genomes of these viruses were sequenced. The assembled sequences were used to define the genotypes of these strains PCR-RFLP methodology was used to distinguish isolates and capture ELISA was used to demonstrate the antigenic changes resulted from ORF2 gene mutation of the isolates. Results We identified 19 PCV2 isolates including four newly emerging PCV2 mutant strains. The 19 isolates were designated into three genotypes PCV2a PCV2b and PCV2d . PCV2d represented a novel genotype and a shift from PCV2a to PCV2b as the predominant genotype in China was identified. This is the first report of 1766 nt PCV2 harboring a base deletion at other new different positions. Amino acid sequence analysis identified two novel ORF2 mutations resulting in ORF2 sequences 705 and 708 nt in length in three deletion strains 1766 nt and one strain with a genome 1767 nt in length. Finding of two amino acids elongation of the ORF2-encoded Cap protein is firstly observed among PCV2 strains all over the world. The isolates were distinguished into different genotypes by PCR-RFLP methodology and antigenic changes were present in Cap protein of mutation isolates by capture eLisa. Conclusions The results of this study provide evidence that PCV2 is undergoing constant genetic variation and that the .