tailieunhanh - Báo cáo y học: " Human parvovirus PARV4 DNA in tissues from adult individuals: a comparison with human parvovirus B19 (B19V)

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Human parvovirus PARV4 DNA in tissues from adult individuals: a comparison with human parvovirus B19 (B19V) | Corcioli et al. Virology Journal 2010 7 272 http content 7 1 272 VIROLOGY JOURNAL RESEARCH Open Access Human parvovirus PARV4 DNA in tissues from adult individuals a comparison with human parvovirus B19 B19V Fabiana Corcioli1 Krystyna Zakrzewska1 Rosa Fanci2 Vincenzo De Giorgi3 Massimo Innocenti4 Matteo Rotellini5 Simonetta Di Lollo5 Alberta Azzi1 Abstract Background PARV4 is a new member of the Parvoviridae family not closely related to any of the known human parvoviruses. Viremia seems to be a hallmark of PARV4 infection and viral DNA persistence has been demonstrated in a few tissues. Till now PARV4 has not been associated with any disease and its prevalence in human population has not been clearly established. This study was aimed to assess the tissue distribution and the ability to persist of PARV4 in comparison to parvovirus B19 B19V . Results PARV4 and B19V DNA detection was carried out in various tissues of individuals without suspect of acute viral infection by a real time PCR and a nested PCR targeting the ORF2 and the ORF1 respectively. Low amount of PARV4 DNA was found frequently 40 in heart and liver of adults individuals less frequently in lungs and kidneys 23 5 and 18 respectively and was rare in bone marrow skin and synovium samples 5 5 4 and 5 respectively . By comparison B19V DNA sequences were present in the same tissues with a higher frequency significantly higher in myocardium skin and bone marrow except than in liver where the frequency was the same of PARV4 DNA and in plasma samples where B19V frequency was significantly lower than that of PARV4 Conclusions The particular tropism of PARV4 for liver and heart here emerged suggests to focus further studies on these tissues as possible target for viral replication and on the possible role of PARV4 infection in liver and heart diseases. Neither bone marrow nor kidney seem to be a common target of viral replication. Background PARV4 is a member of the Parvoviridae family .