tailieunhanh - Báo cáo y học: "Extra-cellular release and blood diffusion of BART viral micro-RNAs produced by EBV-infected nasopharyngeal carcinoma cells"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Extra-cellular release and blood diffusion of BART viral micro-RNAs produced by EBV-infected nasopharyngeal carcinoma cells | Gourzones et al. Virology Journal 2010 7 271 http content 7 1 271 VIROLOGY JOURNAL RESEARCH Open Access Extra-cellular release and blood diffusion of BART viral micro-RNAs produced by EBV-infected nasopharyngeal carcinoma cells z-1 z 1 1 1 1 1 3 4 Claire Gourzones Aurore Gelin Izabela Bombik Jihene Klibi Benjamin Vérillaud Joel Guigay Philippe Lang Stéphane Témam3 Véronique Schneider2 Corinne Amiel2 Sonia Baconnais 1 Anne-Sophie Jimenez1 Pierre Busson1 Abstract Background Nasopharyngeal carcinoma NPC is a human epithelial malignancy consistently associated with the Epstein-Barr virus. The viral genome is contained in the nuclei of all malignant cells with abundant transcription of a family of viral microRNAs called BART miRNAs. MicroRNAs are well known intra-cellular regulatory elements of gene expression. In addition they are often exported in the extra-cellular space and sometimes transferred in recipient cells distinct from the producer cells. Extra-cellular transport of the microRNAs is facilitated by various processes including association with protective proteins and packaging in secreted nanovesicles called exosomes. Presence of microRNAS produced by malignant cells has been reported in the blood and saliva of tumor-bearing patients especially patients diagnosed with glioblastoma or ovarian carcinoma. In this context it was decided to investigate extra-cellular release of BART miRNAs by NPC cells and their possible detection in the blood of NPC patients. To address this question we investigated by quantitative RT-PCR the status of 5 microRNAs from the BART family in exosomes released by NPC cells in vitro as well as in plasma samples from NPC xenografted nude mice and NPC patients. Results We report that the BART miRNAs are released in the extra-cellular space by NPC cells being associated at least to a large extent with secreted exosomes. They are detected with a good selectivity in plasma samples from NPC xenografted nude mice as well as