tailieunhanh - Báo cáo y học: " Inhibitory effect of small interfering RNA on dengue virus replication in mosquito cells"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Inhibitory effect of small interfering RNA on dengue virus replication in mosquito cells | Wu et al. Virology Journal 2010 7 270 http content 7 1 270 VIROLOGY JOURNAL RESEARCH Open Access Inhibitory effect of small interfering RNA on dengue virus replication in mosquito cells Vì Air ì A I1 2 2t I_11 I I I_I f z t 3t 11 V 71 I S-. 1 4 r I I A 11 V I 7r z i I I1 I I I I I s 1 1 I 7 I I I I4 Xinwei wu Hua nong Jinya Yue Yejian wu Xiangzhong LI Liyun Jiang Lei LI Qiaoyan LI Guoquan Gao4 5 Xia Yang2 4 Abstract Background Dengue viruses DENs are the wildest transmitted mosquito-borne pathogens throughout tropical and sub-tropical regions worldwide. Infection with DENs can cause severe flu-like illness and potentially fatal hemorrhagic fever. Although RNA interference triggered by long-length dsRNA was considered a potent antiviral pathway in the mosquito only limited studies of the value of small interfering RNA siRNA have been conducted. Results A 21 nt siRNA targeting the membrane glycoprotein precursor gene of DEN-1 was synthesized and transfected into mosquito C6 36 cells followed by challenge with DEN. The stability of the siRNA in cells was monitored by flow cytometry. The antiviral effect of siRNA was evaluated by measurement of cell survival rate using the MTT method and viral RNA was quantitated with real-time RT-PCR. The presence of cells containing siRNA at 1 3 5 7 days after transfection were and respectively. After 7 days incubation with DEN there was reduced cytopathic effect increased cell survival rate vs and reduced viral RNA copies Ct value vs detected in transfected C6 36 cells. Conclusions Our data showed that synthetic siRNA against the DEN-1 membrane glycoprotein precursor gene effectively inhibited DEN-1 viral RNA replication and increased C6 36 cell survival rate. siRNA may offer a potential new strategy for prevention and treatment of DEN infection. Background Dengue viruses DENs are the wildest transmitted arbovirus members of the family .