tailieunhanh - Báo cáo y học: " Genital herpes evaluation by quantitative TaqMan PCR: correlating single detection and quantity of HSV-2 DNA in cervicovaginal lavage fluids with cross-sectional and longitudinal clinical data"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Genital herpes evaluation by quantitative TaqMan PCR: correlating single detection and quantity of HSV-2 DNA in cervicovaginal lavage fluids with cross-sectional and longitudinal clinical data | Aumakhan et al. Virology Journal 2010 7 328 http content 7 1 328 VIROLOGY JOURNAL RESEARCH Open Access Genital herpes evaluation by quantitative TaqMan PCR correlating single detection and quantity of HSV-2 DNA in cervicovaginal lavage fluids with cross-sectional and longitudinal clinical data 1 2 1 Bulbulgul Aumakhan Andrew Hardick Thomas C Quinn Oliver Laeyendecker Stephen J Gange f hric Ro ror1 rictrinhar r tv1 k f tar n Ar iTiC fT ic4 rihan5 D Ifta l l iOroonHI i i 6 rÌTỉniol I K ioronctoin7 Chiis Beyiei Cinistopiiei Cox Kathiyn Anastos Maidge Cohen Ruth M Gieenblatt Daniel J ivieienstein Howard Minkoff8 Marek Nowicki9 Charlotte A Gaydos2 Abstract Objective To evaluate the utility of a single quantitative PCR qPCR measurement of HSV HSV-1 2 DNA in cervicovaginal lavage CVL specimens collected from women with predominantly chronic HSV-2 infection in assessing genital HSV shedding and the clinical course of genital herpes GH within a cohort with semiannual schedule of follow up and collection of specimens. Methods Two previously described methods used for detection of HSV DNA in mucocutaneous swab samples were adapted for quantification of HSV DNA in CVLs. Single CVL specimens from 509 women were tested. Presence and quantity of CVL HSV DNA were explored in relation to observed cross-sectional and longitudinal clinical data. Results The PCR assay was sensitive and reproducible with a limit of quantification of 50 copies per milliliter of CVL. Overall 7 of the samples were positive for HSV-2 DNA with median log10 HSV-2 DNA copy number of IQR . No HSV-1 was detected. Presence and quantity of HSV-2 DNA in CVL directly correlated with the clinical signs and symptoms of presence of active symptomatic disease with frequent recurrences. Conclusion Single qPCR measurement of HSV DNA in CVL fluids of women with chronic HSV-2 infection provided useful information for assessing GH in the setting of infrequent sampling of specimens. .