tailieunhanh - Báo cáo y học: " Simultaneous detection and differentiation by multiplex real time RT-PCR of highly pathogenic avian influenza subtype H5N1 classic (clade 2.2.1 proper) and escape mutant (clade 2.2.1 variant) lineages in Egypt"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Simultaneous detection and differentiation by multiplex real time RT-PCR of highly pathogenic avian influenza subtype H5N1 classic (clade proper) and escape mutant (clade variant) lineages in Egypt | Abdelwhab et al. Virology Journal 2010 7 260 http content 7 1 260 VIROLOGY JOURNAL METHODOLOGY Open Access Simultaneous detection and differentiation by multiplex real time RT-PCR of highly pathogenic avian influenza subtype H5N1 classic clade proper and escape mutant clade variant lineages in Egypt cl c - r I h z A k I - h A p - k1 2 3 A h z D K 2 K I r f- I K CT KI I K 1 h ỉ ri z 7 ill -1r1A IC- 1-f-- r A 2 K I ỉ r D r r K 1 El-sayed M Abdelwnab AnmeQ M ErTan Christian Grund Mario Ziller Abdel-satar AidTd Martin Deer Mona M Aly2 HaTez M HaTez3 Timm C Harder1 Abstract Background The endemic status oT highly pathogenic avian influenza virus HPAIV oT subtype H5N1 in Egypt continues to devastate the local poultry industry and poses a permanent threat Tor human health. Several genetically and antigenically distinct H5N1 lineages co-circulate in Egypt Strains oT clade proper replicate mainly in backyard birds causing the bulk oT human inTections while a variant lineage within appears to be perpetuated mainly in commercial poultry Tarms in Egypt. Viruses oT the lineage represent driTt variants escaping Trom conventional vaccine-induced immunity and some oT these strains also escaped detection by commercial real time reverse transcriptase PCR RT-qPCR protocols due to mismatches in the primers probe binding sites. Results We developed therefore a versatile sensitive and lineage-speciTic multiplex RT-qPCR Tor detection and typing oTH5N1 viruses in Egypt. Analytical characterization was carried out using 50 Egyptian HPAIV H5N1 strains isolated since 2006 and 45 other avian inTluenza viruses AIV . A detection limit oT400 cRNA copies per ml sample matrix was Tound. Higher diagnostic sensitivity oT the multiplex assay in comparison to other generic H5 or M-gene based RT-qPCR assays were Tound by examination oT 63 swab samples Trom experimentally inTected chickens and 50 AIV-positive swab samples Trom diTTerent host .