tailieunhanh - Báo cáo y học: " Molecular characterization, structural analysis and determination of host range of a novel bacteriophage LSB-1"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Molecular characterization, structural analysis and determination of host range of a novel bacteriophage LSB-1 | Chai et al. Virology Journal 2010 7 255 http content 7 1 255 VIROLOGY JOURNAL RESEARCH Open Access Molecular characterization structural analysis and determination of host range of a novel bacteriophage LSB-1 Yaming Chai Hongyan Xiong Xiangyu Ma Liqing Cheng Guorong Huang Zhonglin Rao Lin Zhang Abstract Background Bacteriophages phages are widespread in the environment and play a crucial role in the evolution of their bacterial hosts and the emergence of new pathogens. Results LSB-1 a reference coliphage strain was classified as a member of the Podoviridae family with a cystic form 50 5 nm diameter and short tail 60 5 nm long . The double stranded DNA was about 30 kilobase pairs in length. We identified its host range and determined the gp17 sequences and protein structure using shotgun analysis and bioinformatics technology. Conclusions Coliphage LSB-1 possesses a tailspike protein with endosialidase activity which is probably responsible for its specific enteroinvasive host range within the laboratory. Background Bacteriophages phages are widespread in the environment and play a crucial role in the evolution of their bacterial hosts and the emergence of new pathogens. They have enormous potential for the development of new drugs therapies and environmental control technologies such as natural non-toxic alternatives for controlling bacterial pathogens. Recent interest in phages has been stimulated by studies that demonstrate the efficacy of phages in preventing and treating infections 1-3 . Phages are readily isolated from water samples in the environment. Some of the isolated phages have shown broad-host range interaction with the bacterial isolates and others have shown either species or strain level specificity. Both polyvalent phages and non-polyvalent phages are morphologically and genetically diverse 4 5 . They are efficient at host recognition but there is no single method of adsorption and different phages employ different .