tailieunhanh - Báo cáo y học: "Evaluation of a rapid diagnostic test, NanoSign® Influenza A/B Antigen, for detection of the 2009 pandemic influenza A/H1N1 viruses"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Evaluation of a rapid diagnostic test, NanoSign® Influenza A/B Antigen, for detection of the 2009 pandemic influenza A/H1N1 viruses | Lee et al. Virology Journal 2010 7 244 http content 7 1 244 VIROLOGY JOURNAL RESEARCH Open Access Evaluation of a rapid diagnostic test NanoSign Influenza A B Antigen for detection of the 2009 pandemic influenza A H1N1 viruses Gyu-Cheol l_eeH Eun-Sung Jeon2t Won-Shik Kim3 Dung Tien Le4 Jong-Ha Yoo5 Chom-Kyu Chong 2 Abstract Background This study evaluated the clinical accuracy and analytical sensitivity of the NanoSign Influenza A B antigen kit in detecting 2009 pandemic influenza A H1N1 viruses. The kit is one of the most popular rapid diagnostic tests for detecting influenza in Republic of Korea. Results The NanoSign Influenza A B kit resulted in sensitivity and specificity compared to RT-PCR in the detection of the viruses from 1 023 specimens. In addition the kit was able to detect two strains of novel influenza viruses Influenza A California 12 2009 H1N1 and clinically isolated wild-type novel influenza A H1N1 both of which are spreading epidemically throughout the world. In addition the correlation between NanoSign Influenza A B test and conventional RT-PCR was approximately 94 indicating a high concordance rate. Analytical sensitivity of the kit was approximately 73 ng mL of the purified viral proteins and hemagglutination units for the cultured virus. Conclusions As the NanoSign Influenza A B kit showed relatively high sensitivity and specificity and the good correlation with RT-PCR it will be very useful in the early control of influenza infection and in helping physicians in making early treatment decisions. Background The novel influenza A H1N1 virus has spread to most of the world s populations and its spread has led to a pandemic alert situation 1-3 . As a result at the end of 2009 the World Health Organization announced that the novel influenza A H1N1 had reached pandemic status 4 . A variety of different diagnostic methods can be used to detect the presence of influenza viruses in respiratory specimens such