tailieunhanh - Báo cáo y học: " Echoviruses are a major cause of aseptic meningitis in infants and young children in Kuwait"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Echoviruses are a major cause of aseptic meningitis in infants and young children in Kuwait | Dalwai et al. Virology Journal 2010 7 236 http content 7 1 236 VIROLOGY JOURNAL RESEARCH Open Access Echoviruses are a major cause of aseptic meningitis in infants and young children in Kuwait Ajmal Dalwai Suhail Ahmad Widad Al-Nakib Abstract Background The etiologic agents of aseptic meningitis AM often include human enteroviruses. The role of enteroviruses causing AM in young children was investigated during a 3-year period in Kuwait. Results Enteroviral RNA was detected in cerebrospinal fluid CSF by reverse transcription-PCR and specific genotypes of enteroviruses were identified by direct DNA sequencing of VP4-VP2 region. Enteroviral RNA was detected in 92 of 387 24 suspected AM cases and the results were confirmed by hybridization of amplicons with an internal enterovirus-specific probe. The CSF samples from 75 of 281 27 children 2 years old but only from 3 of 38 8 4-12 year-old children were positive for enteroviral RNA p . Majority of infections in children 2 years old 49 of 75 65 were due to three echoviruses echovirus type 9 E9 E11 and E30. Only three other enteroviruses namely coxsackievirus type B4 coxsackievirus type B5 and enterovirus 71 were detected among AM cases in Kuwait. Conclusions Our data show that three types of echoviruses E9 E11 and E30 are associated with the majority of AM cases in Kuwait. To the best of our knowledge this is the first report to characterize different enterovirus genotypes associated with AM in the Arabian Gulf region. Introduction Aseptic meningitis AM is a severe potentially fatal infection of the central nervous system CNS and is characterized by meningeal inflammation that is not associated with any identifiable bacterial pathogen in the cerebrospinal fluid CSF 1 . Most patients with AM present with abrupt onset of fever accompanied by complaints of headache stiff neck lethargy anorexia and may also experience vomiting diarrhea sore throat and rash. The CNS involvement in neonates may not be .