tailieunhanh - Báo cáo y học: "Ferrets develop fatal influenza after inhaling small particle aerosols of highly pathogenic avian influenza virus A/Vietnam/1203/2004 (H5N1"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Ferrets develop fatal influenza after inhaling small particle aerosols of highly pathogenic avian influenza virus A/Vietnam/1203/2004 (H5N1 | Lednicky et al. Virology Journal 2010 7 231 http content 7 1 231 VIROLOGY JOURNAL RESEARCH Open Access Ferrets develop fatal influenza after inhaling small particle aerosols of highly pathogenic avian influenza virus A Vietnam 1203 2004 H5N1 1 13 1 1 2 John A Lednicky Sara B Hamilton Richard S Tuttle William A Sosna Deirdre E Daniels David E Swayne Abstract Background There is limited knowledge about the potential routes for H5N1 influenza virus transmission to and between humans and it is not clear whether humans can be infected through inhalation of aerosolized H5N1 virus particles. Ferrets are often used as a animal model for humans in influenza pathogenicity and transmissibility studies. In this manuscript a nose-only bioaerosol inhalation exposure system that was recently developed and validated was used in an inhalation exposure study of aerosolized A Vietnam 1203 2004 H5N1 virus in ferrets. The clinical spectrum of influenza resulting from exposure to A Vietnam 1203 2004 H5N1 through intranasal verses inhalation routes was analyzed. Results Ferrets were successfully infected through intranasal instillation or through inhalation of small particle aerosols with four different doses of Influenza virus A Vietnam 1203 2004 H5N1 . The animals developed severe influenza encephalomyelitis following intranasal or inhalation exposure to 101 102 103 or 104 infectious virus particles per ferret. Conclusions Aerosolized Influenza virus A Vietnam 1203 2004 H5N1 is highly infectious and lethal in ferrets. Clinical signs appeared earlier in animals infected through inhalation of aerosolized virus compared to those infected through intranasal instillation. Background Human infections caused by H5N1 highly pathogenic avian influenza viruses H5N1 that arose from 2003-onwards have been rare as evident by only 500 cases confirmed through 5 July 2010. However H5N1 have a fatality rate of about 59 1 . In ferret transmission models the H5N1 viruses were .