tailieunhanh - Báo cáo hóa học: " Atomic Force Microscopy Study of Protein– Protein Interactions in the Cytochrome CYP11A1 (P450scc)-Containing Steroid Hydroxylase System"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Atomic Force Microscopy Study of Protein– Protein Interactions in the Cytochrome CYP11A1 (P450scc)-Containing Steroid Hydroxylase System | Ivanov et al. Nanoscale Res Lett 2011 6 54 http content 6 1 54 o Nanoscale Research Letters a SpringerOpen Journal NANO EXPRESS Open Access Atomic Force Microscopy Study of ProteinProtein Interactions in the Cytochrome CYP11A1 P450scc -Containing Steroid Hydroxylase System vn 2fìCi 1 PA Rranfci i 7m 1 A 7r nor M 1 A Archakov1 A Ro nkj R Romharrrn I D Ivanov PA Fiarnsuzov A ZUllnei NV Medvedeva AI Aichdkov W Reinle R Beinildidl Abstract Atomic force microscopy AFM and photon cotteldtion spectroscopy PCS were used foi monitoring of the procedure foi cytochrome CYP11A1 monomerizdtion in solution without phospholipids. It was shown that the incubation of 100 pM CYP11A1 with 12 Emulgen 913 in 50 mM KP pH foi 10 min dt T 22 C leads to dissociation of hemopiotein aggregates to monomeis with the monomeiization degree of 82 4 . Following the monomeiization procedure CYP11A1 remained functionally active. AFM was employed to detect and visualize the isolated pioteins as well as complexes foimed between the components of the cytochiome CYP11A1-dependent steioid hydioxylase system. Both Ad and AdR weie present in solution as monomeis. The typical heights of the monomeiic AdR Ad and CYP11A1 images weie measured by AFM and weie found to coiiespond to the sizes nm nm and nm respectively. The binaiy Ad AdR and AdR CYP11A1mon complexes with the heights nm and nm respectively weie registered by use of AFM. The Ad CYP11A1mon complex foimation reaction was kinetically characterized based on optical biosensoi data. In addition the teinaiy AdR Ad CYP11A1 complexes with a typical height of 4 1 nm weie AFM registered. Introduction Hemeproteins belonging to cytochrome P450 superfamily play an important role in metabolism of a broad spectrum of endogenous and exogenous chemicals 1 . CYP11A1-dependent monooxygenase system is responsible for cholesterol conversion to pregnenolone 2 3 . The electron transfer chain of this .