tailieunhanh - báo cáo khoa học:" Short-term effects of amelogenin gene splice products A+4 and A-4 implanted in the exposed rat molar pulp"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Short-term effects of amelogenin gene splice products A+4 and A-4 implanted in the exposed rat molar pulp | Head Face Medicine BioMed Central Research Short-term effects of amelogenin gene splice products A 4 and A-4 implanted in the exposed rat molar pulp Nadège Jegat1 Dominique Septier1 Arthur Veis2 Anne Poliard3 and Michel Goldberg 1 Open Access Address 1Oral Biology EA 2496 Faculté de Chirurgie Dentaire Université Paris Descartes Montrouge France 2Feinberg School of Medicine Northwestern University Chicago USA and 3Laboratoire de Différenciation Cellules Souches et Prions CNRS-FRE2937 Villejuif Cedex France Email Nadège Jegat-nadjeg@ Dominique Septier-dseptier@ Arthur Veis - aveis@ Anne Poliard - apoliard@ Michel Goldberg - mgoldod@ Corresponding author Published 21 December 2007 Received 4 December 2007 . . Accepted 21 December 2007 Head Face Medicine 2007 3 40 doi 1746-160X-3-40 This article is available from http content 3 1 40 2007 Jegat et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract In order to study the short-time effects of two bioactive low-molecular amelogenins A 4 and A-4 half-moon cavities were prepared in the mesial aspect of the first maxillary molars and after pulp exposure agarose beads alone controls or beads soaked in A 4 or A-4 experimental were implanted into the pulp. After l 3 or 7 days the rats were killed and the teeth studied by immunohistochemistry. Cell proliferation was studied by PCNA labeling positive at 3 days but decreasing at day 7 for A 4 whilst constantly high between 3 and 7 days for A-4. The differentiation toward the osteo odontoblast lineage shown by RP59 labeling was more apparent for A-4 compared with A 4. Osteopontin-positive cells were alike at days 3 and 7 for A-4. In contrast for A 4 .

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