tailieunhanh - Báo cáo y học: " Vascular relaxation of canine visceral arteries after ischemia by means of supraceliac aortic cross-clamping followed by reperfusion"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Vascular relaxation of canine visceral arteries after ischemia by means of supraceliac aortic cross-clamping followed by reperfusion | Ci scato et al. Scandinavian Journal ofTrauma Resuscitation and Emergency Medicine 2010 18 41 http content 18 1 41 SCANDINAVIAN JOURNAL OF Et emergency medicine ORIGINAL RESEARCH Open Access Vascular relaxation of canine visceral arteries after ischemia by means of supraceliac aortic cross-clamping followed by reperfusion José G Ciscato Junior Verena KCapellini Andrea C Celotto Caroline F Baldo Edwaldo E Joviliano Paulo RB Evora Marcelo B Dalio and Carlos E Piccinato Abstract Background The supraceliac aortic cross-clamping can be an option to save patients with hipovolemic shock due to abdominal trauma. However this maneuver is associated with ischemia reperfusion I R injury strongly related to oxidative stress and reduction of nitric oxide bioavailability. Moreover several studies demonstrated impairment in relaxation after I R but the time course of I R necessary to induce vascular dysfunction is still controversial. We investigated whether 60 minutes of ischemia followed by 30 minutes of reperfusion do not change the relaxation of visceral arteries nor the plasma and renal levels of malondialdehyde MDA and nitrite plus nitrate NOx . Methods Male mongrel dogs n 27 were randomly allocated in one of the three groups sham no clamping n 9 ischemia supraceliac aortic cross-clamping for 60 minutes n 9 and I R 60 minutes of ischemia followed by reperfusion for 30 minutes n 9 . Relaxation of visceral arteries celiac trunk renal and superior mesenteric arteries was studied in organ chambers. MDA and NOx concentrations were determined using a commercially available kit and an ozone-based chemiluminescence assay respectively. Results Both acetylcholine and calcium ionophore caused relaxation in endothelium-intact rings and no statistical differences were observed among the three groups. Sodium nitroprusside promoted relaxation in endothelium-denuded rings and there were no inter-group statistical differences. Both plasma and renal concentrations of MDA and .