tailieunhanh - Báo cáo y học: "A steady state analysis indicates that negative feedback regulation of PTP1B by Akt elicits bistability in insulin-stimulated GLUT4 translocation"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: A steady state analysis indicates that negative feedback regulation of PTP1B by Akt elicits bistability in insulin-stimulated GLUT4 translocation | Theoretical Biology and Medical Modelling BioMed Central Research Open Access A steady state analysis indicates that negative feedback regulation of PTP1B by Akt elicits bistability in insulin-stimulated GLUT4 translocation Lopamudra Girit Vivek K Mutalikt and KVVenkatesh Address Department of Chemical Engineering and School of Biosciences and Bioengineering Indian Institute of Technology Bombay Powai Mumbai-400076 India Email Lopamudra Giri - lopa@ Vivek K Mutalik - vivekm@ KV Venkatesh - venks@ Corresponding author fEqual contributors Published 03 August 2004 Received 22 June 2004 Theoretical Biology and Medical Modelling 2004 1 2 doi 1742-4682-1-2 Accepted 03 August 2004 This article is available from http content 1 1 2 2004 Giri et al licensee BioMed Central Ltd. This is an open-access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The phenomenon of switch-like response to graded input signal is the theme involved in various signaling pathways in living systems. Positive feedback loops or double negative feedback loops embedded with nonlinearity exhibit these switch-like bistable responses. Such feedback regulations exist in insulin signaling pathway as well. Methods In the current manuscript a steady state analysis of the metabolic insulin-signaling pathway is presented. The threshold concentration of insulin required for glucose transporter GLUT4 translocation was studied with variation in system parameters and component concentrations. The dose response curves of GLUT4 translocation at various concentration of insulin obtained by steady state analysis were quantified in-terms of half saturation constant. Results We show that insulin-stimulated GLUT4 translocation can operate as a .