tailieunhanh - báo cáo khoa học:" Decreased CD90 expression in human mesenchymal stem cells by applying mechanical stimulation"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Decreased CD90 expression in human mesenchymal stem cells by applying mechanical stimulation | Head Face Medicine BioMed Central Research Open Access Decreased CD90 expression in human mesenchymal stem cells by applying mechanical stimulation Anne Wiesmann 1 Hans-Jorg Buhring1 Christoph Mentrup2 and Hans-Peter Wiesmann2 Address 1Medizinische Klinik und Poliklinik Abteilung fur Hamatologie Onkologie Immunologie und Rheumatologie Universitatsklinikum Tubingen Otfried-Muller-Str. 10 D-72076 Tubingen Germany and 2Klinik und Poliklinik fur Mund und Kiefer-Gesichtschirurgie Westfalische Wilhelms-Universitat Munster D-48149 Munster Germany Email Anne Wiesmann - Hans-Jorg Buhring - Christoph Mentrup - mentrup@ Hans-Peter Wiesmann - wiesmap@ Corresponding author Published 31 March 2006 Received 05 February 2006 Head Face Medicine 2006 2 8 doi 1746-I60X-2-8 Accepted 31 March 2006 This article is available from http content 2 1 8 2006 Wiesmann et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Mesenchymal stem cells MSC are multipotent cells which can differentiate along osteogenic chondrogenic and adipogenic lineages. The present study was designed to investigate the influence of mechanical force as a specific physiological stress on the differentiation of MSC to osteoblast-like cells. Methods Human MSC were cultured in osteoinductive medium with or without cyclic uniaxial mechanical stimulation 2000 pstrain 200 cycles per day 1 Hz . Cultured cells were analysed for expression of collagen type I osteocalcin osteonectin and CD90. To evaluate the biomineral formation the content of bound calcium in the cultures was determined. Results After 14 days in culture immunfluorescence