tailieunhanh - Báo cáo y học: "Tyrosine phosphorylation of myosin heavy chain during skeletal muscle differentiation: an integrated bioinformatics approach"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Tyrosine phosphorylation of myosin heavy chain during skeletal muscle differentiation: an integrated bioinformatics approach | Theoretical Biology and Medical Modelling BioMed Central Research Open Access Tyrosine phosphorylation of myosin heavy chain during skeletal muscle differentiation an integrated bioinformatics approach DF Harney RK Butler and RJ Edwards Address Department of Clinical Pharmacology Royal College of Surgeons in Ireland 123 St. Stephens Green Dublin 2 Ireland Email DF Harney - dharney@ RK Butler - ryanbutler@ RJ Edwards - redwards@ Corresponding author Published 25 March 2005 Received 16 January 2005 Theoretical Biology and Medical Modelling 2005 2 12 doi 1742-4682-2-12 Accepted 25 March 2005 This article is available from http content 2 1 12 2005 Harney et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Previously it has been shown that insulin-mediated tyrosine phosphorylation of myosin heavy chain is concomitant with enhanced association of C-terminal SRC kinase during skeletal muscle differentiation. We sought to identify putative site s for this phosphorylation event. Results A combined bioinformatics approach of motif prediction and evolutionary and structural analyses identified tyrosines163 and 1856 of the skeletal muscle heavy chain as the leading candidate for the sites of insulin-mediated tyrosine phosphorylation. Conclusion Our work is suggestive that tyrosine phosphorylation of myosin heavy chain whether in skeletal muscle or in platelets is a significant event that may initiate cytoskeletal reorganization of muscle cells and platelets. Our studies provide a good starting point for further functional analysis of MHC phosphor-signalling events within different cells. Introduction Myosins actin-based motor proteins are expressed as multiple isoforms

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