tailieunhanh - Chapter 065. Gene Therapy in Clinical Medicine (Part 1)

Harrison's Internal Medicine Chapter 65. Gene Therapy in Clinical Medicine Gene Therapy in Clinical Medicine: Introduction Gene transfer is a novel area of therapeutics in which the active agent is a nucleic acid sequence rather than a protein or small molecule. Because delivery of naked DNA or RNA to a cell is an inefficient process, most gene transfer is carried out using a vector, or gene delivery vehicle. These vehicles have generally been engineered from viruses by deleting some or all of the viral genome and replacing it with the therapeutic gene of interest under the control of a suitable. | Chapter 065. Gene Therapy in Clinical Medicine Part 1 Harrison s Internal Medicine Chapter 65. Gene Therapy in Clinical Medicine Gene Therapy in Clinical Medicine Introduction Gene transfer is a novel area of therapeutics in which the active agent is a nucleic acid sequence rather than a protein or small molecule. Because delivery of naked DNA or RNA to a cell is an inefficient process most gene transfer is carried out using a vector or gene delivery vehicle. These vehicles have generally been engineered from viruses by deleting some or all of the viral genome and replacing it with the therapeutic gene of interest under the control of a suitable promoter Table 65-1 . Gene transfer strategies can be described in terms of three essential elements 1 a vector 2 a gene to be delivered and 3 a relevant target cell to which the DNA or RNA is delivered. The series of steps in which the donated DNA enters the target cell and begins expression is referred to as transduction. Gene delivery can take place in vivo in which the vector is directly injected into the patient or in the case of hematopoietic and some other target cells ex vivo with removal of the target cells from the patient followed by return of the modified autologous cells after gene transfer in the laboratory. The latter approach offers opportunities to integrate gene transfer techniques with cellular therapies Chap. 67 . Table 65-1 Characteristics of Gene Delivery Vehicles Viral Vectors Features Retroviral Lentiviral Adenoviral AAV Hun Foamy Vir Viral genome RNA RNA DNA DNA RN Cell division Yes G1 phase No No No Viral Vectors Features Retroviral Lentiviral Adenoviral AAV Hun Foamy Vir requirement Packaging limitation 8 kb 8 kb 8-30 kb 5 kb 1 Immune responses to vector Few Few Extensive Few Few Genome integration Yes Yes Poor Poor Yes Longterm expression Yes Yes No Yes Yes Main Persistent Persistent Highly Elicits .

• Y học thường thức
• Gene Therapy
• Clinical Medicine
• bệnh học và điều trị
• bài giảng bệnh học
• tài liệu y khoa
• Harrisons Internal Medicine
• Transgene Expression
• Uptake Mechanism
• Gene Delivery
• Polylipid Nanoparticle
• Mesenchymal stem cells
• Baculoviral vector
• Cancer gene therapy
• Green fluorescence protein
• BMC Cancer
• Genetically engineered mesenchymal stromal cells
• Cell therapy
• Suicide gene therapy
• Clinical trial
• Clostridium perfringens enterotoxin
• Colon cancer
• Suicide gene
• Bladder cancer
• Radical cystectomy
• Perioperative chemotherapy
• Perioperative targeted therapy
• Immune therapy
• trình bày báo cáo
• tài liệu báo cáo khoa học
• nghiên cứu y học
• báo cáo y học
• kiến thức y học
• Novel
• Gene
• Therapy
• Approaches
• Ming Wei
• David Good
• Dermal fibroblast
• Nucleofection method
• Green fluorescent protein
• Clinical gene therapy
• Fetal Calf Serum
• Adeno associated viral vector
• Chicken beta actin promoter
• Heparan sulfate proteoglycans
• Red fluorescent protein
• Gene transfer techniques
• Hepatocellular carcinoma
• Combined therapy
• VX2 tumor
• Tumor gene therapy
• Thymidine kinase
• Directly targeting
• Bifidobacterial recombinant thymidine kinase
• Targeting system
• Antitumor mechanisms
• Artificial microRNA
• DNA polymerase
• Caspase 3
• Human sodium iodide symporter
• Radioiodine gene therapy
• Adenine nucleotide translocase 2
• Short hairpin RNA
• Radiation induced immune response
• BMC Bioinformatics
• Patient derived xenograft model
• Gene expression
• Predictive cancer biomarker
• Targeted therapy
• Drug response prediction
• Viral insertion
• Viral integration
• Sequence mapping
• Genome viewer
• BMC Molecular and Cell Biology
• Retinal neovascularization
• Endothelial progenitor cells
• Endostatin transfected EPCs
• BMC Musculoskeletal Disorders
• Endothelial mesenchymal transition
• Mouse aortic endothelial cells
• Endothelial cell targeting property
• Colorectal cancer
• MEK inhibitor
• Cancer stem cell
• Gene expression signature
• báo cáo khoa học
• báo cáo hóa học
• công trình nghiên cứu về hóa học
• tài liệu về hóa học
• cách trình bày báo cáo