tailieunhanh - Báo cáo y học: "Effects of hydrogen sulfide on hemodynamics, inflammatory response and oxidative stress during resuscitated hemorrhagic shock in rats"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Effects of hydrogen sulfide on hemodynamics, inflammatory response and oxidative stress during resuscitated hemorrhagic shock in rats. | Ganster et al. Critical Care 2010 14 R165 http content 14 5 R165 c CRITICAL CARE RESEARCH Open Access Effects of hydrogen sulfide on hemodynamics inflammatory response and oxidative stress during resuscitated hemorrhagic shock in rats Frédérique Ganster1 2 6 Mélanie Burban1 Mathilde de la Bourdonnaye1 Lionel Fizanne1 Olivier Douay1 3 4 3 12 Laurent Loufrani Alain Mercat Paul Calès Peter Radermacher Daniel Henrion Pierre Asfar Ferhat Meziani2 5 6 Abstract Introduction Hydrogen sulfide H2S has been shown to improve survival in rodent models of lethal hemorrhage. Conversely other authors have reported that inhibition of endogenous H2S production improves hemodynamics and reduces organ injury after hemorrhagic shock. Since all of these data originate from unresuscitated models and or the use of a pre-treatment design we therefore tested the hypothesis that the H2S donor sodium hydrosulfide NaHS may improve hemodynamics in resuscitated hemorrhagic shock and attenuate oxidative and nitrosative stresses. Methods Thirty-two rats were mechanically ventilated and instrumented to measure mean arterial pressure MAP and carotid blood flow CBF . Animals were bled during 60 minutes in order to maintain MAP at 40 2 mm Hg. Ten minutes prior to retransfusion of shed blood rats randomly received either an intravenous bolus of NaHS mg kg or vehicle NaCl . At the end of the experiment T 300 minutes blood aorta and heart were harvested for Western blot inductible Nitric Oxyde Synthase iNOS Nuclear factor-KB NF-kB phosphorylated Inhibitor kB P-IkB Inter-Cellular Adhesion Molecule I-CAM Heme oxygenase 1 HO-1 Heme oxygenase 2 HO-2 as well as nuclear respiratory factor 2 Nrf2 . Nitric oxide NO and superoxide anion O2- were also measured by electron paramagnetic resonance. Results At the end of the experiment control rats exhibited a decrease in MAP which was attenuated by NaHS 65 32 versus 101 17 mmHg P . CBF was better maintained in NaHS-treated rats .