tailieunhanh - Báo cáo y học: " Increased HMGB1 expression and release by mononuclear cells following"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Increased HMGB1 expression and release by mononuclear cells following . | Manganelli et al. Critical Care 2010 14 R197 http content 14 6 R197 c CRITICAL CARE RESEARCH Open Access Increased HMGB1 expression and release by mononuclear cells following surgical anesthesia trauma 1 2 3 1 3 Valeria Manganelli Michele Signore Ilaria Pacini Roberta Misasi Guglielmo Tellan Tina Garofalo 1 Emanuela Lococo1 Piero Chirletti5 Maurizio Sorice1 4 f Giovanna Delogu3t Abstract Introduction High mobility group box 1 HMGB1 is a key mediator of inflammation that is actively secreted by macrophages and or passively released from damaged cells. The proinflammatory role of HMGB1 has been demonstrated in both animal models and humans since the severity of inflammatory response is strictly related to serum HMGB1 levels in patients suffering from traumatic insult including operative trauma. This study was undertaken to investigate HMGB1 production kinetics in patients undergoing major elective surgery and to address how circulating mononuclear cells are implicated in this setting. Moreover we explored the possible relationship between HMGB1 and the proinflammatory cytokine interleukin-6 IL-6 . Methods Forty-seven subjects American Society of Anesthesiologists physical status I and II scheduled for major abdominal procedures were enrolled. After intravenous medication with midazolam mg Kg all patients received a standard general anesthesia protocol by thiopentone sodium 5 mg Kg and fentanyl pg Kg plus injected Vecuronium mg Kg . Venous peripheral blood was drawn from patients at three different times t0 before surgery t1 immediately after surgical procedure t2 at 24 hours following intervention. Monocytes were purified by incubation with anti-CD14-coated microbeads followed by sorting with a magnetic device. Cellular localization of HMGB1 was investigated by flow cytometry assay HMGB1 release in the serum by Western blot. Serum samples were tested for IL-6 levels by ELISA. A one-way repeated-measures analysis ANOVA was performed to .