tailieunhanh - Báo cáo y học: " Assessment of monocytic HLA-DR expression in ICU patients: analytical issues for multicentric flow cytometry studie"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Assessment of monocytic HLA-DR expression in ICU patients: analytical issues for multicentric flow cytometry studies. | Monneret et al. Critical Care 2010 14 432 http content 14 4 432 CRITICAL CARE LETTER L_ Assessment of monocytic HLA-DR expression in ICU patients analytical issues for multicentric flow cytometry studies Guillaume Monneret 1 Fabienne Venet1 Christian Meisel2 and Joerg C Schefold3 See related research article by Gogos etal. http content 14 3 R96 We read with interest the recent report by Gogos and colleagues 1 . While the rationale for their study is excellent we would like to comment on technical issues that may have influenced the results. As stated a time limit of 8 hours between sample drawing and staining at a central laboratory was specified 1 . Unfortunately information regarding transport conditions is missing average time temperature . This seems important since monocytic HLA-DR expression mHLA-DR increases artificially over time 2 3 . Consequently recommendations suggest that sample staining for mHLA-DR should occur within 4 hours 2 3 . Although the authors aimed to address the effect of transportation they inappropriately used samples presenting with already near-maximal mHLA-DR values 90 before storage. We therefore assume that mHLA-DR results may be falsely elevated due to prolonged transportation times. Furthermore mHLA-DR modulation during sepsis takes days and consecutive measurements are required 4 . Assessment of one early sample within the first 24 hours is probably inappropriate to investigate the impact of infection on mHLA-DR. Similarly apoptosis staining should not be performed after 8 hours and experts recommendations highlight the need for dedicated protocols on fresh cells 5 . We are convinced that successful future trials in sepsis will rely on our capacity to accurately assess immune responses. In that sense flow cytometry multicentric clinical studies are essential. Such trials should be performed in standardized environments in accordance with specific pre analytical requirements. Otherwise Correspondence .