tailieunhanh - Báo cáo y học: "Upregulation of the pro-apoptotic genes BID and FAS in septic shock patients"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Upregulation of the pro-apoptotic genes BID and FAS in septic shock patients. | Turrel-Davin et al. Critical Care 2010 14 R133 http content 14 4 R133 c CRITICAL CARE RESEARCH Open Access Upregulation of the pro-apoptotic genes BID and FAS in septic shock patients 1 s I z . 2 1 Fanny Turrel-Davin Caroline Guignant Alain Lepape Bruno Mougin Guillaume Monneret Fabienne Venet 2 Abstract Introduction Lymphocyte apoptosis has been suggested to play a central role in sepsis pathophysiology and studies in animal models demonstrated that blocking this pathway improves outcome. However no routine biomarkers of apoptosis are so far available in patients. Thus the aim of our study was to assess the different biomarkers of apoptosis putatively usable on a routine basis in septic shock. Methods Thirteen septic shock patients sampled twice between days 1 to 2 and days 3 to 5 after diagnosis of shock and 15 sex-matched and age-matched healthy controls were prospectively enrolled. Apoptosis was measured in lymphocyte subpopulations using flow cytometry Annexin-V binding activated caspase-3 and Bcl-2 expressions . Representative pro-apoptotic and anti-apoptotic gene expressions were assessed by quantitative reverse-transcription PCR. Monocyte HLA-DR expression and lymphocyte subpopulation cell counts were measured as markers of sepsis-induced immune dysfunctions. To test for statistical significance the Mann-Whitney U test was used with correction by the number of tests performed. Results Flow cytometric measurements of apoptosis in septic shock patients showed an increased Annexin-V binding on CD4 T cells and an increased active caspase-3 expression on B cells only at days 3 to 5 sixfold change and twofold change respectively . Gene expression analysis showed an increased BCL-XL mRNA and an upregulation of the pro-apoptotic genes BID and FAS in septic shock patients 10-fold change and fivefold change respectively compared with healthy controls. Conclusions The present study highlights the difficulties encountered in monitoring apoptosis on