tailieunhanh - Báo cáo y học: "Matrix-metalloproteinase-2, -8 and -9 in serum and skin blister fluid in patients with severe sepsis"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Matrix-metalloproteinase-2, -8 and -9 in serum and skin blister fluid in patients with severe sepsis. | Găddnăs et al. Critical Care 2010 14 R49 http content 14 2 R49 c CRITICAL CARE RESEARCH Open Access Matrix-metalloproteinase-2 -8 and -9 in serum and skin blister fluid in patients with severe sepsis Fiia P Găddnăs 1 Meeri M Sutinen2 Marjo Koskela1 Taina Tervahartiala3 Timo Sorsa3 Tuula A Salo2 Jouko J Laurila1 Vesa Koivukangas4 Tero I Ala-Kokko41 and Aarne Oikarinen45 Abstract Introduction Matrix metalloproteinases MMPs have various roles in inflammatory states. They seem to be able to modulate endothelial barriers and regulate the activity of chemokines and cytokines. The timely development of the levels during severe sepsis and thereafter have not been investigated. In addition it was of interest to study alterations of MMP-levels in intact skin as the skin is the largest barrier against external pathogens and MMPs have not been studied at organ level in human sepsis. The aim of this study was to investigate the timely development of serum and skin MMP-2 -8 and -9 levels in human severe sepsis and their association with disease severity and mortality. Methods Forty-four patients with severe sepsis and fifteen healthy controls were included in this prospective longitudinal study. The amounts of MMP-2 -8 and -9 were analyzed from serum at days 1 4 6 8 and 10 and from skin suction blister fluid at days 1 and 5 from the beginning of severe sepsis. Additionally samples from the survivors were obtained after three and six months. Results The levels of MMP-2 and -8 were up-regulated in severe sepsis in comparison to healthy controls in skin blister fluid and serum. Compared to the controls MMP-9 levels were lower in sepsis from the fourth day on in serum and both the first and fifth day in skin blister fluid. Active forms of MMP-2 and -9 were present only in severe sepsis. The nonsurvivors had higher pro- and active MMP-2 levels than the survivors in skin blister fluid samples. Furthermore MMP-2 levels were more pronounced in blister fluid and serum samples