tailieunhanh - Báo cáo y học: "Renal hypoperfusion and impaired endothelium-dependent vasodilation in an animal model of VILI: the role of the peroxynitrite-PARP pathwa"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Renal hypoperfusion and impaired endothelium-dependent vasodilation in an animal model of VILI: the role of the peroxynitrite-PARP pathway. | Vaschetto et al. Critical Care 2010 14 R45 http content 14 2 R45 c CRITICAL CARE RESEARCH Open Access Renal hypoperfusion and impaired endothelium-dependent vasodilation in an animal model of VILI the role of the peroxynitrite-PARP pathway Rosanna Vaschetto 1 2 3 4 Jan W Kuiper2 4 René JP Musters4 5 Etto C Eringa4 5 Francesco Della Corte 1 Kanneganti Murthy6 AB Johan Groeneveld3 4 and Frans B Plotz2 4 Abstract Introduction Mechanical ventilation MV can injure the lungs and contribute to an overwhelming inflammatory response leading to acute renal failure ARF . We previously showed that poly adenosine diphosphate-ribose polymerase PARP is involved in the development of ventilator-induced lung injury VILI and the related ARF but the mechanisms underneath remain unclear. In the current study we therefore tested the hypothesis that renal blood flow and endothelial functional and tissue changes in the kidney of rats with lipopolysaccharide LPS -induced lung injury aggravated by MV is caused in part by activation of PARP by peroxynitrite. Methods Anesthetized Sprague Dawley rats n 31 were subjected to intratracheal instillation of lipopolysaccharide at 10 mg kg followed by 210 min of mechanical ventilation at either low tidal volume 6 mL kg with 5 cm H2O positive end-expiratory pressure or high tidal volume 19 mL kg with zero positive end-expiratory pressure in the presence or absence of a peroxynitrite decomposition catalyst WW85 or a PARP inhibitor PJ-34. During the experiment hemodynamics and blood gas variables were monitored. At time t t 0 and t 180 min renal blood flow was measured. Blood and urine were collected for creatinine clearance measurement. Arcuate renal arteries were isolated for vasoreactivity experiment and kidneys snap frozen for staining. Results High tidal volume ventilation resulted in lung injury hypotension renal hypoperfusion and impaired renal endothelium-dependent vasodilation associated with renal dysfunction and tissue changes .