tailieunhanh - Báo cáo y học: "The relationship between CD4+CD25+CD127regulatory T cells and inflammatory response and outcome during shock states"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: The relationship between CD4+CD25+CD127regulatory T cells and inflammatory response and outcome during shock states. | Hein et al. Critical Care 2010 14 R19 http content 14 1 R19 c CRITICAL CARE RESEARCH Open Access The relationship between CD4 CD25 CD127 regulatory T cells and inflammatory response and outcome during shock states Frangois Hein1 Frédéric Massin2 Aurélie Cravoisy-Popovic1 Damien Barraud1 Bruno Levy3 Pierre-Edouard Bollaert1 Sébastien Gibot1 3 Abstract Introduction Although regulatory T lymphocytes Tregs have a pivotal role in preventing autoimmune diseases and limiting chronic inflammatory conditions they may also block beneficial immune responses by preventing sterilizing immunity to certain pathogens. Methods To determine whether naturally occurring Treg cells have a role in inflammatory response and outcome during shock state we conducted an observational study in two adult ICUs from a university hospital. Within 12 hours of admission peripheral whole blood was collected for the measurement of cytokines and determination of lymphocyte count. Sampling was repeated at day three five and seven. Furthermore an experimental septic shock was induced in adult Balb c mice through caecal ligation and puncture. Results Forty-three patients suffering from shock 26 septic 17 non septic and 7 healthy volunteers were included. The percentage of Tregs increased as early as 3 days after the onset of shock while their absolute number remained lower than in healthy volunteers. A similar pattern of Tregs kinetics was found in infected and non infected patients. Though there was an inverse correlation between severity scores and Tregs percentage the time course of Tregs was similar between survivors and non survivors. No relation between Tregs and cytokine concentration was found. In septic mice although there was a rapid increase in Treg cells subset among splenocytes antibody-induced depletion of Tregs before the onset of sepsis did not alter survival. Conclusions These data argue against a determinant role of Tregs in inflammatory response and outcome during shock .