tailieunhanh - Báo cáo y học: " Identification of novel conserved functional motifs across most Influenza A viral strains"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Identification of novel conserved functional motifs across most Influenza A viral strains | ElHefnawi et al. Virology Journal 2011 8 44 http content 8 1 44 J VIROLOGY JOURNAL RESEARCH Open Access Identification of novel conserved functional motifs across most Influenza A viral strains I A l A AVA AA I IZ I c I I_I AA A I I 1 1 2 4 r .-. L-YA A Al Al 4 3l l 4il- K l AA 1-A A AVA A 4 2 l ỉ ZA .-A A IZ -A AVA A J2 I ZZA A .-A cl A Fa4 c I I 1-A z 7 A 4 J2 5 D AAaI A Ci A AVA 2 5 Mahmoud tineinawi Osama AlAidi iNdTisa Monameu Mona Kamar Iman tl-Azab Suher zada Kania Siam Abstract Background Influenza A virus poses a continuous threat to global public health. Design of novel universal drugs and vaccine requires a careful analysis of different strains of Influenza A viral genome from diverse hosts and subtypes. We performed a systematic in silico analysis of Influenza A viral segments of all available Influenza A viral strains and subtypes and grouped them based on host subtype and years isolated and through multiple sequence alignments we extrapolated conserved regions motifs and accessible regions for functional mapping and annotation. Results Across all species and strains 87 highly conserved regions conservation percentage 90 and 19 functional motifs conservation percentage 100 were found in PB2 PB1 PA NP M and NS segments. The conservation percentage of these segments ranged between 94 - 98 in human strains the most conserved 85 - 93 in swine strains the most variable and 91 - 94 in avian strains. The most conserved segment was different in each host PB1 for human strains NS for avian strains and M for swine strains . Target accessibility prediction yielded 324 accessible regions with a single stranded probability of which 78 coincided with conserved regions. Some of the interesting annotations in these regions included sites for protein-protein interactions the RNA binding groove and the proton ion channel. Conclusions The influenza virus has evolved to adapt to its host through variations in the GC content and conservation .