tailieunhanh - Báo cáo y học: " In-vitro antiviral activity of Solanum nigrum against Hepatitis C Virus"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: In-vitro antiviral activity of Solanum nigrum against Hepatitis C Virus | Javed et al. Virology Journal 2011 8 26 http content 8 1 26 J VIROLOGY JOURNAL RESEARCH Open Access In-vitro antiviral activity of Solanum nigrum against Hepatitis C Virus Tariq Javed11 Usman Ali Ashfaqrt Sana Riaz2 Sidra Rehman 1 Sheikh Riazuddin3 Abstract Background Hepatitis C is a major health problem causes liver cirrhosis hepatocellular carcinoma and death. The current treatment of standard interferon in combination with ribavirin has limited benefits due to emergence of resistant mutations during long-term treatment adverse side effects and high cost. Hence there is a need for the development of more effective less toxic antiviral agents. Results The present study was designed to search anti-HCV plants from different areas of Pakistan. Ten medicinal plants were collected and tested for anti-HCV activity by infecting the liver cells with HCV 3a innoculum. Methanol and chloroform extracts of Solanum nigrum SN seeds exhibited 37 and more than 50 inhibition of HCV respectively at non toxic concentration. Moreover antiviral effect of SN seeds extract was also analyzed against HCV NS3 protease by transfecting HCV NS3 protease plasmid into liver cells. The results demonstrated that chloroform extract of SN decreased the expression or function of HCV NS3 protease in a dose- dependent manner and GAPDH remained constant. Conclusion These results suggest that SN extract contains potential antiviral agents against HCV and combination of SN extract with interferon will be better option to treat chronic HCV. Introduction An estimated 3 of the world s population 270 million people is chronically infected by HCV which is the main cause of liver fibrosis and cirrhosis that leads to hepatocellular carcinoma HCC in a significant number of patients 1 2 . Almost 10 million people in Pakistan are living with HCV 3 and the most prevalent HCV genotype is 3a followed by 3b and 1a 4 . HCV is enveloped positive strand RNA genome comprising kb of uncapped RNA 5 6