tailieunhanh - Báo cáo y học: " Immunofluorescence Analysis of Duck plague virus gE protein on DPV-infected ducks"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Immunofluorescence Analysis of Duck plague virus gE protein on DPV-infected ducks | Chang et al. Virology Journal 2011 8 19 http content 8 1 19 J VIROLOGY JOURNAL RESEARCH Open Access Immunofluorescence Analysis of Duck plague virus gE protein on DPV-infected ducks 1 2 12 2 2 Hua Chang Anchun Cheng Mingshu Wang Renyong Jia Dekang Zhu Qihui Luo Zhenli Chen Yi Zhou2 Fei Liu2 Xiaoyue Chen1 2 3 Abstract Background In previous studies the expression and localization characteristics of duck plague virus DPV gE protein have been described in cultured cells but the properties of DPV gE protein have not been reported in vivo. Immunofluorescence analysis had been used for the detection of virus antigen but there was no report on the use of this technique for the detection of DPV gE. In this study we investigated the distribution of DPV gE protein on DPV-infected ducks using polyclonal antibody raised against the recombinant His-gE fusion protein by indirect immunofluorescence assay IFA . Results The recombinant gE protein was highly immunogenicity by ELISA and the gE was used as an antigen for the preparation of polyclonal antibody which could be used the first antibody for further experiment to study the distribution of DPV gE protein in DPV-infected tissues by indirect immunofluorescence assay. DPV gE protein were distributed in the immune organs thymus bursa of fabricius BF Harders glands spleen the digestive organs liver duodenum jejunum ileum and the other parenchymatous organs kidney myocardium cerebrum and lung of DPV-infected ducks but the positive immunofluorescence signal was not seen in the muscle and pancreas. The lymphocytes reticulum cells macrophages epithelial cells and hepatocytes served as the principal site for the localization of DPV gE antigen. Moreover the intensity of fluorescence increased sharply from 12 to 216 h post-infection . . Conclusions In this work the immunogenicity of the recombinant gE protein was analyzed by ELISA and we presented the distribution properties of DPV gE antigen in infected .