tailieunhanh - Báo cáo y học: " Hepatitis C virus genotypes circulating in district Swat of Khyber Pakhtoonkhaw, Pakistan"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Hepatitis C virus genotypes circulating in district Swat of Khyber Pakhtoonkhaw, Pakistan | Inamullah et al. Virology Journal 2011 8 16 http content 8 1 16 J VIROLOGY JOURNAL RESEARCH Open Access Hepatitis C virus genotypes circulating in district Swat of Khyber Pakhtoonkhaw Pakistan 1 24 1 1 223 Inamullah Muhammad Idrees Habib Ahmed Sajid-ul-Ghafoor Muhammad Ali Liaqat Ali Aziz Ahmed Abstract Hepatitis C virus HCV is the leading cause of chronic hepatitis worldwide and its subtypes genotypes are clinically important for clinical management and vaccine development. The present study describes frequency distribution of different HCV genotypes and their treatment status in HCV RNA positive patients from district Swat. A total of 185 HCV infected sera were analyzed by molecular genotyping assay. The most prevalent genotype was 3a followed by 2a 3b 7 and 1a . The samples found untypable by the present method of genotypes was while patients with mixed genotype infections were . More than 80 of untypable cases were from those HCV patients who had received interferon plus ribavirin standard therapy in the past and either were non-responders and were relapsed thereafter or were under treatment. In conclusion genotype 3a is the most prevalent HCV genotype in the region. A high prevalence rate of untypable genotypes is present in treated patients that need further investigation for the successful genotyping by developing new assays or using viral sequencing method. Background Hepatitis C virus HCV is a positive single strand RNA virus of Flaviviridae family 1 2 that is approximately Kb in length having a 5 non-coding region 5 NCR a long open reading frame ORF encoding a polyprotein precursor of 3 000 amino acids and 3 NCR. Functions of 5 NCR as an internal ribosomal entry site IRES is important for cap-independent translation of the viral RNA 3 . The virus infect hepatocytes and B lymphocytes of human cells 4 5 and produce 10 trillion virus particles per day even in the late phase of infection 1 . Viral infection is the .