tailieunhanh - Báo cáo y học: " Human herpesvirus 6 major immediate early promoter has strong activity in T cells and is useful for heterologous gene expression"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Human herpesvirus 6 major immediate early promoter has strong activity in T cells and is useful for heterologous gene expression | Matsuura et al. Virology Journal 2011 8 9 http content 8 1 9 J VIROLOGY JOURNAL RESEARCH Open Access Human herpesvirus 6 major immediate early promoter has strong activity in T cells and is useful for heterologous gene expression 1 1 Masaaki Matsuura Masaya Takemoto Koichi Yamanishi Yasuko Mori Abstract Background Human herpesvirus-6 HHV-6 is a beta-herpesvirus. HHV-6 infects and replicates in T cells. The HHV-6-encoded major immediate early gene MIE is expressed at the immediate-early infection phase. Human cytomegalovirus major immediate early promoter CMV MIEp is commercially available for the expression of various heterologous genes. Here we identified the HHV-6 MIE promoter MIEp and compared its activity with that of CMV MIEp in various cell lines. Methods The HHV-6 MIEp and some HHV-6 MIEp variants were amplified by PCR from HHV-6B strain HST. These fragments and CMV MIEp were subcloned into the pGL-3 luciferase reporter plasmid and subjected to luciferase reporter assay. In addition to investigate whether the HHV-6 MIEp could be used as the promoter for expression of foreign genes in a recombinant varicella-zoster virus we inserted HHV-6 MIEp-DsRed expression casette into the varicella-zoster virus genome. Results HHV-6 MIEp showed strong activity in T cells compared with CMV MIEp and the presence of intron 1 of the MIE gene increased its activity. The NF-KB-binding site which lies within the R3 repeat was critical for this activity. Moreover the HHV-6 MIEp drove heterologous gene expression in recombinant varicella-zoster virus-infected cells. Conclusions These data suggest that HHV-6 MIEp functions more strongly than CMV MIEp in various T-cell lines. Background Human herpesvirus 6 HHV-6 was first isolated in 1986 from the peripheral blood of patients with lymphoproliferative disorders and AIDS 1 2 . The virus was subsequently shown to be ubiquitous in healthy adults 3 . HHV-6 has been isolated from infants with exanthema subitum a