tailieunhanh - Báo cáo y học: " Differences in the ability to suppress interferon b production between allele A and allele B NS1 proteins from H10 influenza A viruses"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Differences in the ability to suppress interferon b production between allele A and allele B NS1 proteins from H10 influenza A viruses | Zohari et al. Virology Journal 2010 7 376 http content 7 1 376 J VIROLOGY JOURNAL RESEARCH Open Access Differences in the ability to suppress interferon b production between allele A and allele B NS1 proteins from H10 influenza A viruses 1 1 1 Siamak Zohari Muhammad Munir Giorgi Metreveli Sándor Belák Mikael Berg Abstract Background In our previous study concerning the genetic relationship among H10 avian influenza viruses with different pathogenicity in mink Mustela vison we found that these differences were related to amino acid variations in the NS1 protein. In this study we extend our previous work to further investigate the effect of the NS1 from different gene pools on type I IFN promoter activity the production of IFN-b as well as the expression of the IFN-b mRNA in response to poly I C. Results Using a model system we first demonstrated that NS1 from A mink Sweden 84 H10N4 allele A could suppress an interferon-stimulated response element ISRE reporter system to about 85 . The other NS1 allele B from A chicken Germany N 49 H10N7 was also able to suppress the reporter system but only to about 20 . The differences in the abilities of the two NS1s from different alleles to suppress the ISRE reporter system were clearly reflected by the protein and mRNA expressions of IFN-b as shown by ELISA and RT-PCR assays. Conclusions These studies reveal that different non-structural protein 1 NS1 of influenza viruses one from allele A and another from allele B show different abilities to suppress the type I interferon b expression. It has been hypothesised that some of the differences in the different abilities of the alleles to suppress ISRE were because of the interactions and inhibitions at later stages from the IFN receptor such as the JAK STAT pathway. This might reflect the additional effects of the immune evasion potential of different NS1s. Background Type I interferons IFNs play an essential role in both the innate immune response and the