tailieunhanh - Báo cáo y học: " Transient expression of bC1 protein differentially regulates host genes related to stress response, chloroplast and mitochondrial functions"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Transient expression of bC1 protein differentially regulates host genes related to stress response, chloroplast and mitochondrial functions | Andleeb et al. Virology Journal 2010 7 373 http content 7 1 373 J VIROLOGY JOURNAL RESEARCH Open Access Transient expression of pci protein differentially regulates host genes related to stress response chloroplast and mitochondrial functions Saiqa Andleeb Imran Amin Aftab Bashir Rob W Briddon Shahid Mansoor Abstract Background Geminiviruses are emerging plant pathogens that infect a wide variety of crops including cotton cassava vegetables ornamental plants and cereals. The geminivirus disease complex consists of monopartite begomoviruses that require betasatellites for the expression of disease symptoms. These complexes are widespread throughout the Old World and cause economically important diseases on several crops. A single protein encoded by betasatellites termed pC1 is a suppressor of gene silencing inducer of disease symptoms and is possibly involved in virus movement. Studies of the interaction of pC1 with hosts can provide useful insight into virus-host interactions and aid in the development of novel control strategies. We have used the differential display technique to isolate host genes which are differentially regulated upon transient expression of the pC1 protein of chili leaf curl betasatellite ChLCB in Nicotiana tabacum. Results Through differential display analysis eight genes were isolated from Nicotiana tabacum at two and four days after infitration with pC1 of ChLCB expressed under the control of the Cauliflower mosaic virus 35S promoter. Cloning and sequence analysis of differentially amplified products suggested that these genes were involved in ATP synthesis and acted as electron carriers for respiration and photosynthesis processes. These differentially expressed genes DEGs play an important role in plant growth and development cell protection defence processes replication mechanisms and detoxification responses. Kegg orthology based annotation system analysis of these DEGs demonstrated that one of the genes coding for .