tailieunhanh - Báo cáo y học: " Chicken cyclophilin A is an inhibitory factor to influenza virus replication"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Chicken cyclophilin A is an inhibitory factor to influenza virus replication | Xu et al. Virology Journal 2010 7 372 http content 7 1 372 RESEARCH J VIROLOGY JOURNAL Open Access Chicken cyclophilin A is an inhibitory factor to influenza virus replication Chongfeng Xu1 Shanshan Meng1 Xiaoling Liu2 Lei Sun2 Wenjun Liu2 3 Abstract Background The importance of enhancing influenza resistance in domestic flocks is quite clear both scientifically and economically. Chicken is very susceptible to influenza virus. It has been reported that human cellular cyclophilin A CypA impaired influenza virus infection in 293T cells. Whether chicken CypA chCypA inhibits influenza virus replication is not known. The molecular mechanism of resistance in chicken to influenza virus remains to be studied. Results The chCypA gene was isolated and characterized in the present study. It contained an ORF of 498 bp encoding a polypeptide of 165 amino acids with an estimated molecular mass of kDa sharing high identity with mammalian CypA genes. The chCypA demonstrated an anti-influenza activity as expected. ChCypA protein was shown to be able to specifically interact with influenza virus M1 protein. Cell susceptibility to influenza virus was reduced by over-expression of chCypA in CEF cells. The production of recombinant influenza virus A WSN 33 reduced to one third in chCypA expressing cells comparing to chCypA absent cells. ChCypA was widely distributed in a variety of chicken tissues. It localized in cytoplasm of chicken embryo fibroblast CEF cells. Avian influenza virus infection induced its translocation from cytoplasm into nucleus. ChCypA expression was not significantly up-regulated by avian influenza virus infection. The present study indicated that chCypA was an inhibitory protein to influenza virus replication suggesting a role as an intrinsic immunity factor against influenza virus infection. Conclusion The present data demonstrates that chCypA possesses anti-influenza virus activity which allows the consideration of genetic improvement for