tailieunhanh - Báo cáo y học: " A novel protein-coding ORF72.2 gene was identified from Marek’s disease virus strain CVI988"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: A novel protein-coding gene was identified from Marek’s disease virus strain CVI988 | Tian et al. Virology Journal 2010 7 371 http content 7 1 371 J VIROLOGY JOURNAL SHORT REPORT Open Access A novel protein-coding gene was identified from Marek s disease virus strain CVI988 1 X 71 111 1 r- s Mingxing Tian Yang Zhao Min Shi Yan Lin Nianli Zou Ping Liu Xintian Wen Sanjie Cao Yong Huang1 2 Abstract Marek s disease is a highly contagious disease of poultry characterized by rapid-on set of T-cell lymphomas which is caused by Marek s disease virus MDV but its pathogenic mechanism is still not very clear. Recently some new progress were achieved in molecular character of MDV. Along with the genomic sequencing of MDV serotype 1 some novel open reading frames ORFs were predicted and was one of them which have no homologues in other MDV serotypes or in other alphaherpesvirus. In the study was firstly identified as a proteincoding gene by the method of reverse transcription polymerase chain reaction RT-PCR western blotting and indirect immunofluorescence assay. This study paved the way to conduct further studies to determine whether plays a role in MDV replication and pathogenicity. Findings Marek s disease MD is a highly contagious disease of poultry characterized by mononuclear cellular infiltrates in peripheral nerves and various other organs and tissues including iris and skin. The disease has a worldwide distribution and remains a major concern for the poultry industry even though vaccines are used widely today 1 . The causative agent of the disease is Gallid herpesvirus 2 which is also called Marek s disease virus MDV serotype 1. This virus is an alphaherpesvirus of the genus Mardivirus 2 which also includes the antigen-related Meleagrid herpesvirus 1 HVT a strain used widely as a vaccine against MD since the late 1960s 3 4 as well as Gallid herpesvirus 3 MDV-2 which includes apathogenic strains some of which are used as live vaccines against MD 5 . The complete genomic sequence of the MDV-1 .