tailieunhanh - Báo cáo y học: " Soluble CD40 ligand-activated human peripheral B cells as surrogated antigen presenting cells: A preliminary approach for anti-HBV immunotherapy"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Soluble CD40 ligand-activated human peripheral B cells as surrogated antigen presenting cells: A preliminary approach for anti-HBV immunotherapy | Wu et al. Virology Journal 2010 7 370 http content 7 1 370 J VIROLOGY JOURNAL RESEARCH Open Access Soluble CD40 ligand-activated human peripheral B cells as surrogated antigen presenting cells A preliminary approach for anti-HBV immunotherapy 1 2 z- - 7 1 1 f Z-I 1 2 w 2 3 Chao Wu Yong Liu Qi Zhao Guangmei Chen Junhao Chen Xiaomin Yan Yi-Hua Zhou 1 Zuhu Huang Abstract Background We aimed to clarify whether soluble CD40 ligand sCD40L activated B cells may be loaded with HBcAg18-27 peptide and served as antigen-producing cells APCs to induce HBV-specific cytolytic T lymphocytes CTLs . Results Human B cells could be cultured in the presence of sCD40L up to 54 days and the proportion of B cells in the S phase increased from 0 to in the culture. The expression of CD80 CD86 major histocompatibility complex MHC classes I and II molecules on the sCD40L-activated B cell was significantly increased after long-time culture. Cytometry and fluorescence microscopy showed that more than 98 sCD40L-activated B cells were loaded by the HBcAg peptide. Furthermore the peptide-pulsed activated B cells could induce HBcAg18-27 specific CTLs. Conclusions Our results demonstrate that sCD40L-activated B cells may function as APCs and induce HBV-specific CTLs. Background Efficient antigen presentation by antigen presenting cells APCs is critical for inducing T-cell mediated immunity in vivo 1 2 . Dendritic cells DCs activated macrophages and activated B cells are all capable of presenting antigen peptides. DCs are considered to be highly efficient at antigen capture processing and migration 3 . Therefore DCs have been used to generate antigenspecific T cells for immunotherapy 4-6 . Recently it has been demonstrated that B cells may function as APCs 1 7 in addition to the essential role in the humoral immune response. Banchereau et al first reported the CD40 system 8 and suggested to use CD40 ligand CD40L stimulated B cells as an alternative or complementary APC. .