tailieunhanh - Báo cáo y học: " Production and purification of VP2 protein of porcine parvovirus expressed in an insectbaculovirus cell system"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Production and purification of VP2 protein of porcine parvovirus expressed in an insectbaculovirus cell system | Zhou et al. Virology Journal 2010 7 366 http content 7 1 366 J VIROLOGY JOURNAL SHORT REPORT Open Access Production and purification of VP2 protein of porcine parvovirus expressed in an insect-baculovirus cell system Hongchao Zhou1t Guizhe Yao2t Shangjin Cui 2 1 Abstract The porcine parvovirus PPV VP2 protein was expressed in an insect-baculovirus cell system and was purified using Ni-NTA affinity column chromatography. The recombinant 6-His-tagged VP2 protein with molecular mass Mr of about 64 kDa was detected by anti-his antibody and anti-PPV serum. Electron microscopy showed that the purified VP2 protein assembled into spherical particles with diameters ranging from 20 to 22 nm. The expressed VP2 was antigenically similar to the native capsid protein according to HA and a Western blotting assay performed with polyclonal antibodies collected from an outbreak of PPV in one farm. This study provides a foundation for the application of VP2 protein in the clinical diagnosis of PPV or in the vaccination against PPV in the future. 1. Introduction Porcine parvovirus PPV causes reproductive failure in pregnant sows. The infection occurs without clinical symptoms in adults however the virus can cross the placental barrier during the infection and cause the death of the fetuses stillbirths and return to estrus 1 . More recently PPV has gained importance as an agent able to increase the effects of porcine circovirus type 2 infection in the clinical course of postweaning multisystemic wasting syndrome PMWS 2 3 which is a significant disease in global swine production 4 . Widespread vaccination has been proposed as a cost-effective method to reduce the economical losses due to the endemic and worldwide prevalence of this virus 5 6 . PPV is a small non-enveloped single-stranded negative-sense DNA virus. Capsids of PPV are assembled from three viral proteins VP1 VP2 and VP3 . The major structural protein VP2 is the main target for neutralizing antibodies in .