tailieunhanh - Báo cáo y học: "Real-time reverse-transcription PCR in the diagnosis of influenza A (H1N1)v in intensive care unit adult patients"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Real-time reverse-transcription PCR in the diagnosis of influenza A (H1N1)v in intensive care unit adult patients. | Available online http content 13 6 428 Letter Real-time reverse-transcription PCR in the diagnosis of influenza A H1N1 v in intensive care unit adult patients Concepcion Gimeno1 2 and David Navarro2 3 Microbiology Service Consorcio Hospital General Universitario Spain 2Department of Microbiology School of Medicine Valencia Spain 3Microbiology Service Hospital Clínico Universitario Spain Corresponding author David Navarro Published 7 December 2009 This article is online at http content 13 6 428 2009 BioMed Central Ltd Critical Care 2009 13 428 doi cc8164 See related research by Rello et al. http content 13 5 R148 Regarding the interesting article on novel influenza A H1N1 v infection in intensive care adult patients with severe respiratory failure recently published in Critical Care 1 we should like to make the following comments. The authors found that real-time RT-PCR for novel influenza A H1N1 v virus in nasopharyngeal swabs on intensive care unit ICU admission was negative in four patients who later had a positive PCR result in respiratory secretions obtained at intubation and concluded that a negative PCR result at admission should not exclude influenza A H1N1 v due to the presence of false negative results in at least 10 cases 1 . We of course understand what the authors meant. In our opinion however this assumption is formally inaccurate and is somewhat misleading as it might be inferred that real-time RT-PCR gives at least 10 of false negative results in patients with overt symptomatic influenza requiring ICU admission. The negative results reported by the authors cannot be considered true false negative RT-PCR results as samples were not tested in parallel by a different assay yielding a positive result - in fact RT-PCR which was used at participating centers is currently the standard method for the diagnosis of influenza. Optimal sensitivity of RT-PCR and rapid antigen tests is achieved .

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