tailieunhanh - Báo cáo khoa học: Identification of malic and soluble oxaloacetate decarboxylase enzymes in Enterococcus faecalis

Two paralogous genes,maeEandcitM, that encode putative malic enzyme family members were identified in the Enterococcus faecalisgenome. MaeE (41 kDa) and CitM (42 kDa) share a high degree of homology between them (47% identities and 68% conservative substitutions). However, the genetic context of each gene suggested thatmaeEis associated with malate utilization whereascitM is linked to the citrate fermentation pathway. | 1FEBS Journal Identification of malic and soluble oxaloacetate decarboxylase enzymes in Enterococcus faecalis Martin Espariz1 Guillermo Repizo1 Victor Blancato1 Pablo Mortera2 Sergio Alarcon2 and Christian Magni1 1 Institute de Biologia Molecular y Celular de Rosario IBR-CONICET Universidad Nacionalde Rosario Argentina 2 Instituto de Quimica de Rosario IQUIR-CONICET Universidad Nacionalde Rosario Argentina Keywords citrate metabolism Enterococcus faecalis malate metabolism malic enzyme oxaloacetate decarboxylase Correspondence C. Magni Instituto de Biologia Molecular y Celular de Rosario IBR Suipacha 531 Rosario Santa Fe Argentina Fax 54 341 439 0465 Tel 54 341 435 0661 E-mail magni@ Received 17 February 2011 revised 7 April 2011 accepted 19 April 2011 doi Two paralogous genes maeE and citM that encode putative malic enzyme family members were identified in the Enterococcus faecalis genome. MaeE 41 kDa and CitM 42 kDa share a high degree of homology between them 47 identities and 68 conservative substitutions . However the genetic context of each gene suggested that maeE is associated with malate utilization whereas citM is linked to the citrate fermentation pathway. In the present work we focus on the biochemical characterization and physiological contribution of these enzymes in E. faecalis. With this aim the recombinant versions of the two proteins were expressed in Escherichia coli affinity purified and finally their kinetic parameters were determined. This approach allowed us to establish that MaeE is a malate oxidative decarboxylating enzyme and CitM is a soluble oxaloacetate decarboxylase. Moreover our genetic studies in E. faecalis showed that the citrate fermentation phenotype is not affected by citM deletion. On the other hand maeE gene disruption resulted in a malate fermentation deficient strain indicating that MaeE is responsible for malate metabolism in E. faecalis. Lastly it was demonstrated that malate .

• Báo cáo khoa học
• Scientific reports
• biochemistry
• molecular Biology
• Enterococcus faecalis
• decarboxylase
• oxaloacetate
• BMC Cancer
• Glutamate oxaloacetate transaminase 1
• Redox regulation
• Metabolic regulation
• KRAS mutated cancer cells
• Report medicine
• traditional medicine
• oxidation
• Crystal structure
• bacteria
• breast cancer
• biological activities
• environmental medicine
• BMC Biotechnology
• Aspergillus carbonarius
• Mitochondrial transport protein
• Citric acid
• Malic acid
• Metabolic engineering
• Non small cell lung cancer
• Cisplatin resistance
• Increase cisplatin sensitivity
• Circular RNAs