tailieunhanh - Báo cáo khoa học: Nautilin-63, a novel acidic glycoprotein from the shell nacre of Nautilus macromphalus

In molluscs, and more generally in metazoan organisms, the production of a calcified skeleton is a complex molecular process that is regulated by the secretion of an extracellular organic matrix. This matrix constitutes a cohe-sive and functional macromolecular assemblage, containing mainly pro-teins, glycoproteins and polysaccharides that, together, control the biomineral formation. | IFEBS Journal Nautilin-63 a novel acidic glycoprotein from the shell nacre of Nautilus macromphalus Benjamin Marie1 2 Isabelle Zanella-Cleon3 Marion Corneillat4 Michel Becchi3 Gerard Alcaraz2 4 Laurent Plasseraud2 5 Gilles Luquet1 2 and Frederic Marin1 2 1 UMR 5561 CNRS Biogeosciences Dijon France 2 Universite de Bourgogne Dijon France 3 IFR 128 BioSciences UMR 5086 CNRS IBCP Universite de Lyon 1 Lyon France 4 UPSP PROXISS Departement Agronomie Environnement AgroSup Dijon France 5 ICMUB UMR CNRS 5260 Faculte des Sciences Mirande Dijon France Keywords biomineralization de novo sequencing immunolocalization mollusc shell nacre organic matrix Correspondence B. Marie or F. Marin UMR 5561 CNRS Biogeosciences Universite de Bourgogne 6 Boulevard GabrielDijon 21000 France Fax 33 3 80 39 63 87 Tel 33 3 80 39 63 72 E-mail Received 29 September 2010 revised 18 March 2011 accepted 11 April 2011 doi In molluscs and more generally in metazoan organisms the production of a calcified skeleton is a complex molecular process that is regulated by the secretion of an extracellular organic matrix. This matrix constitutes a cohesive and functional macromolecular assemblage containing mainly proteins glycoproteins and polysaccharides that together control the biomineral formation. These macromolecules interact with the extruded precursor mineral ions mainly calcium and bicarbonate to form complex organo-mineral composites of well-defined microstructures. For several reasons related to its remarkable mechanical properties and to its high value in jewelry nacre is by far the most studied molluscan shell microstructure and constitutes a key model in biomineralization research. To understand the molecular mechanism that controls the formation of the shell nacreous layer we have investigated the biochemistry of Nautilin-63 one of the main nacre matrix proteins of the cephalopod Nautilus macromphalus.