tailieunhanh - Báo cáo khoa học: Specific biomarkers for stochastic division patterns and starvation-induced quiescence under limited glucose levels in fission yeast

Glucose as a source of energy is centrally important to our understanding of life. We investigated the cell division–quiescence behavior of the fission yeastSchizosaccharomyces pombeunder a wide range of glucose concentra-tions (0–111 mM). The mode of S. | IFEBS Journal Specific biomarkers for stochastic division patterns and starvation-induced quiescence under limited glucose levels in fission yeast Tomas Pluskal1 Takeshi Hayashi1 2 Shigeaki Saitoh3 Asuka Fujisawa2 and Mitsuhiro Yanagida1 2 1 Okinawa Institute of Science and Technology Promotion Corporation Okinawa Japan 2 CREST Research Project Japan Science and Technology Corporation JST Graduate Schoolof Biostudies Kyoto University Japan 3 Division of CellBiology Institute of Life Science Kurume University Fukuoka Japan Keywords CDP-choline ergothioneine glutathione longevity trehalose Correspondence M. Yanagida CREST Research Project Japan Science and Technology Corporation JST Graduate Schoolof Biostudies Kyoto University Sakyo-ku Kyoto 606-8501 Japan Fax 81 75 753 4208 Tel 81 75 753 4205 E-mail yanagida@ Present address Kashiwa Chuo High School Chiba Japan Re-use of this article is permitted in accordance with the Terms and Conditions set out at http onlineopen OnlineOpen_Terms Received 14 October 2010 revised 1 January 2011 accepted 7 February 2011 doi Glucose as a source of energy is centrally important to our understanding of life. We investigated the cell division-quiescence behavior of the fission yeast Schizosaccharomyces pombe under a wide range of glucose concentrations 0-111 mM . The mode of S. pombe cell division under a microfluidic perfusion system was surprisingly normal under highly diluted glucose concentrations mM 1 20 of the standard medium within human blood sugar levels . Division became stochastic accompanied by a curious division-timing inheritance in mM glucose. A critical transition from division to quiescence occurred within a narrow range of concentrations mM . Under starvation mM conditions cells were mostly quiescent and only a small population of cells divided. Under fasting 0 mM conditions division was immediately arrested with a .