tailieunhanh - Báo cáo y học: "DHEA-dependent and organ-specific regulation of TNF-α mRNA expression in a murine polymicrobial sepsis and trauma model"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: DHEA-dependent and organ-specific regulation of TNF-α mRNA expression in a murine polymicrobial sepsis and trauma model. | Available online http content 13 4 R114 Open Access Research DHEA-dependent and organ-specific regulation of TNF-a mRNA expression in a murine polymicrobial sepsis and trauma model Tanja Barkhausen1 Frank Hildebrand1 Christian Krettek1 and Martijn van Griensven2 Department of Trauma Surgery Hannover Medical School Carl-Neuberg-Strasse 1 D-30625 Hannover Germany 2Ludwig Boltzmann Institute for Experimental and Clinical Traumatology AUVA Research Center Donaueschingenstrasse 13 A-1200 Vienna Austria Corresponding author Tanja Barkhausen Received 30 Jan 2009 Revisions requested 24 Mar 2009 Revisions received 18 May 2009 Accepted 13 Jul 2009 Published 13 Jul 2009 Critical Care 2009 13 R11 4 doi cc7963 This article is online at http content 13 4 R114 2009 Barkhausen et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Introduction Dehydroepiandrosterone DHEA improves survival after trauma and sepsis while mechanisms of action are not yet fully understood. Therefore we investigated the influence of DHEA on local cytokine expression in a two-hit model. Methods Male NMRI mice were subjected to femur fracture hemorrhagic shock and subsequent sepsis. Sham-operated animals were used as controls. DHEA 25 mg kg or vehicle was administered daily. Mortality rate activity and body temperature were determined daily after sepsis induction. TNF-a IL-1P and IL-10 mRNA expression pattern were investigated in lung and liver tissue after 48 and 96 hours. Results DHEA treatment resulted in a significantly reduced mortality rate and improvements in the clinical status. On cytokine level only TNF-a was significantly reduced in the cecal ligation and puncture CLP -vehicle .