tailieunhanh - Báo cáo " Using improved Non Ri -Maprec assay to detect virulence mutations in poliomyelitis Vaccine viruses: advantages over rct40 test "

Mutant analysis by polymerase chain reaction and restriction enzyme cleavage (MAPREC) has been developed by Chumakov et al. for poliovirus to determine quantitatively the presence of genomic changes in particular nucleotide sequences that lead to virulence phenotypes of oral poliomyelitis vaccine (OPV or live attenuated vaccine) [1]. Afterwards, this RI (radioisotope) MAPREC was developed to non RI – MAPREC by Japan Poliomyelitis Research Institute in 1998 to compensate for some disadvantages of RI-MAPREC [2]. This test was highly recommended for laboratories to assess the safety of their vaccine products. At Poliovac, the rct40 test was used to be considered a major tool for vaccine products examination. In. | VNU Journal of Science Natural Sciences and Technology 23 2007 145-151 Using improved Non Ri -Maprec assay to detect virulence mutations in poliomyelitis Vaccine viruses advantages over rct40 test Thieu Thi Hoai Anh Le Thi Luan Poliomyelitis vaccine Research and Production center Poliovac 135 Lo Duc Hanoi Vietnam Received 23 January 2006 Abstract. Mutant analysis by polymerase chain reaction and restriction enzyme cleavage MAPREC has been developed by Chumakov et al. for poliovirus to determine quantitatively the presence of genomic changes in particular nucleotide sequences that lead to virulence phenotypes of oral poliomyelitis vaccine OPV or live attenuated vaccine 1 . Afterwards this RI radioisotope MAPREC was developed to non RI - MAPREC by Japan Poliomyelitis Research Institute in 1998 to compensate for some disadvantages of RI-MAPREC 2 . This test was highly recommended for laboratories to assess the safety of their vaccine products. At Poliovac the rct40 test was used to be considered a major tool for vaccine products examination. In this research we have improved the non RI MAPREC test to apply it well with practical conditions in our laboratory and compared this test to the usual rct40 test to point out its great advantages. 1. Introduction Poliomyelitis is an acute disease of the central nervous system CNS caused by poliovirus. Oral poliomyelitis vaccine OPV is usually considered the major tool for eradication of poliomyelitis in many countries as well as Vietnam. It is regarded as one of the safest vaccines in current use but some polio vaccine related - paralytic cases have been reported recently 3 . These cases are caused by vaccine viruses undergoing reverted mutations during repeated replications. Poliovirus has a genome of single-stranded RNA its rate of mutations is usually high during replication since RNA-dependent RNA polymerase does not have the proof - reading Corresponding author. Tel. 84-4-8531213. E-mail tthanh@ activity. Thus

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