tailieunhanh - Báo cáo y học: "Variance in multiplex suspension array assays: intraplex method improves reliability"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: "Variance in multiplex suspension array assays: intraplex method improves reliability | Theoretical Biology and Medical Modelling BioMed Central Research Open Access Variance in multiplex suspension array assays intraplex method improves reliability Brian Hanley 1 2 Address Microbiology Graduate Group University of California Davis CA 95616 USA and 2BW Education and Forensics 2710 Thomes Avenue Cheyenne Wyoming 82001 USA Email Brian Hanley - bphanley@ Published 29 August 2007 Received 6 August 2006 Theoretical Biology and Medical Modelling 2007 4 32 doi 1742-4682-4-32 Accepted 29 August 2007 This article is available from http content 4 1 32 2007 Hanley licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Flow cytometry based suspended microarray assays are susceptible to many sources of variance multi-well replication and inter-instrument reproducibility is uncertain. Method and results An intraplex method was developed in order to minimize differences in sample readings between instruments. A full intraplex assay consists of a set m of microparticle set classifications assaying for the same analyte with each of the m classifier sets having different sensitivity to analyte and n classifier sets replicating each of the m levels of sensitivity where m 1 generally m 4 would be used . Conclusion The intraplex method can compensate adequately for the sources of variance that have been identified in suspended microarray assays. It requires no changes to current equipment in use and is a superior method of constructing precision assays. Additionally Luminex users may want to consider the evidence that shows that despite calibration to the same standard two instruments may not give similar results for all concentrations of analytes. Background A suspended microarray .

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