tailieunhanh - Báo cáo y học: " Variance in multiplex suspension array assays: microsphere size variation impact"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: "Variance in multiplex suspension array assays: microsphere size variation impact | Theoretical Biology and Medical Modelling BioMed Central Research Open Access Variance in multiplex suspension array assays microsphere size variation impact Brian P Hanley 1 Li Xing2 and R Holland Cheng2 Address Microbiology Graduate Group University of California Davis CA 95616 USA and 2Department of Molecular and Cellular Biology University of California Davis CA 95616 USA Email Brian P Hanley - bphanley@ Li Xing - lxing@ R Holland Cheng - rhch@ Corresponding author Published 23 August 2007 Received 15 October 2006 Theoretical Biology and Medical Modelling 2007 4 3 I doi 1742-4682-4-31 Accepted 23 August 2007 This article is available from http content 4 1 31 2007 Hanley et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Luminex suspension microarray assays are in widespread use. There are issues of variability of assay readings using this technology. Methods and results Size variation is demonstrated by transmission electron microscopy. Size variations of microspheres are shown to occur in stepwise increments. A strong correspondence between microsphere size distribution and distribution of fluorescent events from assays is shown. An estimate is made of contribution of microsphere size variation to assay variance. Conclusion A probable significant cause of variance in suspended microsphere assay results is variation in microsphere diameter. This can potentially be addressed by changes in the manufacturing process. Provision to users of mean size median size skew the number of standard deviations that half the size range represents sigma multiple and standard deviation is recommended. Establishing a higher sigma multiple for microsphere .