tailieunhanh - Báo cáo y học: " Computational investigation of epithelial cell dynamic phenotype in vitro"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: "Computational investigation of epithelial cell dynamic phenotype in vitro | Theoretical Biology and Medical Modelling BioMed Central Research Computational investigation of epithelial cell dynamic phenotype in vitro Sean HJ Kim1 Sunwoo Park2 Keith Mostov3 Jayanta Debnath4 and C Anthony Hunt 1 2 Open Access Address 1UCSF UC Berkeley Joint Graduate Group in Bioengineering University of California Berkeley California 94720 USA 2Department of Bioengineering and Therapeutic Sciences University of California San Francisco California 94143 USA 3Department of Anatomy University of California San Francisco California 94143 USA and 4Department of Pathology University of California San Francisco California 94143 USA Email Sean HJ Kim - seanhjk@ Sunwoo Park - parks@ Keith Mostov - Jayanta Debnath - C Anthony Hunt - Corresponding author Published 28 May 2009 Received 8 March 2009 Theoretical Biology and Medical Modelling 2009 6 8 doi 1742-4682-6-8 Accepted 28 May 2009 This article is available from http content 6 1 8 2009 Kim et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background When grown in three-dimensional 3D cultures epithelial cells typically form cystic organoids that recapitulate cardinal features of in vivo epithelial structures. Characterizing essential cell actions and their roles which constitute the system s dynamic phenotype is critical to gaining deeper insight into the cystogenesis phenomena. Methods Starting with an earlier in silico epithelial analogue ISEAI that validated for several Madin-Darby canine kidney MDCK epithelial cell culture attributes we built a revised analogue